Microbial enzymes—an overview

RS Singh, T Singh, A Pandey - Advances in enzyme technology, 2019 - Elsevier
People have exploited the biocatalytic potential of microorganisms for centuries to produce
wine, vinegar, bread, and so forth without understanding their biochemical basis. Microbial …

Computer-Aided Lipase Engineering for Improving Their Stability and Activity in the Food Industry: State of the Art

W Cheng, B Nian - Molecules, 2023 - mdpi.com
As some of the most widely used biocatalysts, lipases have exhibited extreme advantages in
many processes, such as esterification, amidation, and transesterification reactions, which …

Effects of Alcaligenes sp. on corrosion inhibition of sulfate-reducing bacteria in oilfield produced water

J Wang, P Shi, M Du, T Xu, X Shan, Z Tian - Corrosion Science, 2024 - Elsevier
In this study, the effect of Alcaligenes sp. on the corrosion inhibition of sulfate-reducing
bacteria (SRB) was evaluated and the mechanism was discussed from the aspects of …

Effect of Alcaligenes sp. on corrosion behavior of X65 steel in simulated offshore oilfield-produced water

P Shi, M Du, J Wang - Frontiers in Microbiology, 2023 - frontiersin.org
In this paper, the effect of Alcaligenes sp. on the corrosion process of X65 steel was
investigated by using non-targeted metabolomics techniques for comprehensive …

Fitting replacement of signal peptide for highly efficient expression of three penicillin G acylases in E. coli

X Pan, Q Yu, J Chu, T Jiang, B He - Applied microbiology and …, 2018 - Springer
High level expression of penicillin G acylase (PGA) in Escherichia coli is generally
constricted by a complex maturation process and multiple limiting steps. In this study, three …

Small design from big alignment: engineering proteins with multiple sequence alignment as the starting point

T Wang, C Liang, Y Hou, M Zheng, H Xu, Y An… - Biotechnology …, 2020 - Springer
Multiple sequence alignment (MSA) is a fundamental way to gain information that cannot be
obtained from the analysis of any individual sequence included in the alignment. It provides …

Protein engineering of penicillin acylase

VI Tishkov, SS Savin, AS Yasnaya - Acta Naturae (англоязычная …, 2010 - cyberleninka.ru
Penicillin acylases (PA) are widely used for the production of semi-synthetic β-lactam
antibiotics and chiral compounds. In this review, the latest achievements in the production of …

Study cases of enzymatic processes

S Barberis, F Guzmán, A Illanes, J López-Santín… - Enzyme biocatalysis …, 2008 - Springer
Peptides are heteropolymers composed by amino acid residues linked by peptidic bonds
between the carboxyl group of one amino acid residue and the α-amino group of the next …

Industrial and therapeutic enzymes: penicillin acylase

A Illanes, P Valencia - Current developments in biotechnology and …, 2017 - Elsevier
Penicillin acylase is one of the most studied enzymes because of its industrial significance,
but also for being a model for study of prokaryotic expression control and posttranslational …

Optimization of a medium composition for the heterologous production of Alcaligenes faecalis penicillin G acylase in Bacillus megaterium

W Lopes, P Deolindo, AA de Souza Costa… - Protein Expression and …, 2023 - Elsevier
Penicillin G acylase (PGA) is a strategic enzyme in the production processes of beta-lactam
antibiotics. High demand for β-lactam semisynthetic antibiotics explain the genetic and …