Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martínez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Chemical and physical Chitosan modification for designing enzymatic industrial biocatalysts: How to choose the best strategy?

YL Nunes, FL de Menezes, IG de Sousa… - International Journal of …, 2021 - Elsevier
Chitosan is one of the most abundant natural polymer worldwide, and due to its inherent
characteristics, its use in industrial processes has been extensively explored. Because it is …

Anti-biofilm and wound-healing activity of chitosan-immobilized Ficin

DR Baidamshina, VA Koroleva, EY Trizna… - International Journal of …, 2020 - Elsevier
Biofouling is among the key factors slowing down healing of acute and chronic wounds.
Here we report both anti-biofilm and wound-healing properties of the chitosan-immobilized …

Biochemical properties and anti-biofilm activity of chitosan-immobilized papain

DR Baidamshina, VA Koroleva, SS Olshannikova… - Marine Drugs, 2021 - mdpi.com
Chitosan, the product of chitin deacetylation, is an excellent candidate for enzyme
immobilization purposes. Here we demonstrate that papain, an endolytic cysteine protease …

Enzyme immobilization onto renewable polymeric matrixes: Past, present, and future trends

CS Bezerra, CMG de Farias Lemos… - Journal of Applied …, 2015 - Wiley Online Library
In this review, we present an overview of the different renewable polymers that are currently
being used as matrixes for enzyme immobilization and their properties and of new …

Use of chitosan heterofunctionality for enzyme immobilization: β-galactosidase immobilization for galacto-oligosaccharide synthesis

P Urrutia, C Bernal, L Wilson, A Illanes - International journal of biological …, 2018 - Elsevier
Chitosan partially functionalized with aldehyde groups was used for enzyme immobilization,
favoring first the enzyme adsorption through its amino groups and then the covalent bonding …

Immobilization of lipases in hydrophobic chitosan for selective hydrolysis of fish oil: The impact of support functionalization on lipase activity, selectivity and stability

P Urrutia, R Arrieta, L Alvarez, C Cardenas… - International journal of …, 2018 - Elsevier
The objective of this paper was to carry out an integral study of the use of hydrophobic
chitosan as a low-cost support for immobilizing lipases and their further application in the …

Biocatalysis in the winemaking industry: Challenges and opportunities for immobilized enzymes

C Ottone, O Romero, C Aburto… - … reviews in food …, 2020 - Wiley Online Library
Enzymes are powerful catalysts already being used in a large number of industrial
processes. Impressive advantages in enzyme catalysts improvement have occurred in …

Batch synthesis of galactooligosaccharides from co-products of milk processing using immobilized β-galactosidase from Bacillus circulans

CR Hackenhaar, LS Spolidoro, EEE Flores… - Biocatalysis and …, 2021 - Elsevier
Milk whey (MW) and milk whey permeate (MWP) constitute the main part of co-products
derived from milk processing. The use of the transgalactosylation activity of β-galactosidases …

Entrapment of enzyme aggregates in chitosan beads for aroma release in white wines

L Tavernini, C Ottone, A Illanes, L Wilson - International journal of biological …, 2020 - Elsevier
Glycosidases are enzymes involved in the cascade reactions leading to the release of
aromatic compounds in white wines. However, the use of commercial soluble glycosidases …