[HTML][HTML] Protein immobilization techniques for microfluidic assays
Microfluidic systems have shown unequivocal performance improvements over conventional
bench-top assays across a range of performance metrics. For example, specific advances …
bench-top assays across a range of performance metrics. For example, specific advances …
Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics
Biosensing and diagnostic platforms with high sensitivity, specificity, and fast response time
are based on immobilized biomolecules such as antibodies (Abs), aptamers, enzymes …
are based on immobilized biomolecules such as antibodies (Abs), aptamers, enzymes …
Organs-on-chips: breaking the in vitro impasse
AD Van Der Meer, A Van Den Berg - Integrative Biology, 2012 - academic.oup.com
In vitro models of biological tissues are indispensable tools for unraveling human physiology
and pathogenesis. They usually consist of a single layer of a single cell type, which makes …
and pathogenesis. They usually consist of a single layer of a single cell type, which makes …
Using physiologically-based pharmacokinetic-guided “body-on-a-chip” systems to predict mammalian response to drug and chemical exposure
The continued development of in vitro systems that accurately emulate human response to
drugs or chemical agents will impact drug development, our understanding of chemical …
drugs or chemical agents will impact drug development, our understanding of chemical …
Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning
DC Appleyard, SC Chapin, RL Srinivas, PS Doyle - Nature protocols, 2011 - nature.com
This protocol describes the core methodology for the fabrication of bar-coded hydrogel
microparticles, the capture and labeling of protein targets and the rapid microfluidic …
microparticles, the capture and labeling of protein targets and the rapid microfluidic …
[HTML][HTML] Separation and purification of biomacromolecules based on microfluidics
Separation and purification of biomacromolecules either in biopharmaceuticals and fine
chemicals manufacturing, or in diagnostics and biological characterization, can substantially …
chemicals manufacturing, or in diagnostics and biological characterization, can substantially …
The intriguing landscape of single‐cell protein analysis
H Xie, X Ding - Advanced Science, 2022 - Wiley Online Library
Profiling protein expression at single‐cell resolution is essential for fundamental biological
research (such as cell differentiation and tumor microenvironmental examination) and …
research (such as cell differentiation and tumor microenvironmental examination) and …
Synergies between hyperpolarized NMR and microfluidics: a review
Hyperpolarized nuclear magnetic resonance and lab-on-a-chip microfluidics are two
dynamic, but until recently quite distinct, fields of research. Recent developments in both …
dynamic, but until recently quite distinct, fields of research. Recent developments in both …
Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip
Quantitative detection of low abundance proteins is of significant interest for biological and
clinical applications. Here we report an integrated microfluidic solid-phase ELISA platform …
clinical applications. Here we report an integrated microfluidic solid-phase ELISA platform …
Automated microfluidic protein immunoblotting
This protocol describes regional photopatterning of polyacrylamide gels in glass microfluidic
devices as a platform for seamless integration of multiple assay steps. The technology …
devices as a platform for seamless integration of multiple assay steps. The technology …