Publisher Summary This chapter discusses the principles and protocols involved in
mutagenesis of Caenorhabditis elegans. Intended as a guide to the choice of mutagens and …

In C. elegans, mutants that are defective in muscle function and/or structure are easy to
detect and analyze since: 1) body wall muscle is essential for locomotion, and 2) muscle …

Experimental introduction of RNA into cells can be used in certain biological systems to
interfere with the function of an endogenous gene,. Such effects have been proposed to …

A genetic interference phenomenon in the nematode Caenorhabditis elegans has been
described in which expression of an individual gene can be specifically reduced by …

We have investigated the role of trigger RNA amplification during RNA interference (RNAi)
in Caenorhabditis elegans. Analysis of small interfering RNAs (siRNAs) produced during …

Genome editing based on CRISPR (clustered regularly interspaced short palindromic
repeats)-associated nuclease (Cas9) has been successfully applied in dozens of diverse …

A process is provided of introducing an RNA into a living cell to inhibit gene expression of a
target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a …

Graphical dot-matrix plots can provide the most complete and detailed comparison of two
sequences. Presented here is DOTTER2, a dot-plot program for X-windows which can …

Introduction of exogenous double-stranded RNA (dsRNA) into Caenorhabditis elegans has
been shown to specifically and potently disrupt the activity of genes containing homologous …

Hypertrophic cardiomyopathy (HCM) is characterized by ventricular hypertrophy
accompanied by myofibrillar disarrays. Molecular genetic analyses have revealed that …