The mammalian endoplasmic reticulum-associated degradation system
JA Olzmann, RR Kopito… - Cold Spring Harbor …, 2013 - cshperspectives.cshlp.org
The endoplasmic reticulum (ER) is the site of synthesis for nearly one-third of the eukaryotic
proteome and is accordingly endowed with specialized machinery to ensure that proteins …
proteome and is accordingly endowed with specialized machinery to ensure that proteins …
Applying antibodies inside cells: principles and recent advances in neurobiology, virology and oncology
To interfere with cell function, many scientists rely on methods that target DNA or RNA due to
the ease with which they can be applied. Proteins are usually the final executors of function …
the ease with which they can be applied. Proteins are usually the final executors of function …
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit
G Petris, A Casini, C Montagna, F Lorenzin… - Nature …, 2017 - nature.com
In vivo application of the CRISPR-Cas9 technology is still limited by unwanted Cas9
genomic cleavages. Long-term expression of Cas9 increases the number of genomic loci …
genomic cleavages. Long-term expression of Cas9 increases the number of genomic loci …
Tay–Sachs disease mutations in HEXA target the α chain of hexosaminidase A to endoplasmic reticulum–associated degradation
Loss of function of the enzyme β-hexosaminidase A (HexA) causes the lysosomal storage
disorder Tay–Sachs disease (TSD). It has been proposed that mutations in the α chain of …
disorder Tay–Sachs disease (TSD). It has been proposed that mutations in the α chain of …
Conformational targeting of intracellular Aβ oligomers demonstrates their pathological oligomerization inside the endoplasmic reticulum
Aβ oligomers (AβOs) are crucially involved in Alzheimer's Disease (AD). However, the lack
of selective approaches for targeting these polymorphic Aβ assemblies represents a major …
of selective approaches for targeting these polymorphic Aβ assemblies represents a major …
The VCP/p97 and YOD1 proteins have different substrate-dependent activities in endoplasmic reticulum-associated degradation (ERAD)
Endoplasmic reticulum-associated degradation (ERAD) is an essential quality control
mechanism of the folding state of proteins in the secretory pathway that targets …
mechanism of the folding state of proteins in the secretory pathway that targets …
CD4 and BST-2/tetherin proteins retro-translocate from endoplasmic reticulum to cytosol as partially folded and multimeric molecules
CD4 and BST-2/Tetherin are cellular membrane proteins targeted to degradation by the HIV-
1 protein Vpu. In both cases proteasomal degradation following recruitment into the ERAD …
1 protein Vpu. In both cases proteasomal degradation following recruitment into the ERAD …
New tags for recombinant protein detection and O-glycosylation reporters
G Petris, M Bestagno, F Arnoldi, OR Burrone - PloS one, 2014 - journals.plos.org
Monoclonal antibodies (mAbs), because of their unique specificity, are irreplaceable tools
for scientific research. Precise mapping of the antigenic determinants allows the …
for scientific research. Precise mapping of the antigenic determinants allows the …
Reduction of HIV-1 infectivity through endoplasmic reticulum-associated degradation-mediated Env depletion
A Casini, M Olivieri, L Vecchi, OR Burrone… - Journal of …, 2015 - Am Soc Microbiol
During the HIV-1 replicative cycle, the gp160 envelope is processed in the secretory
pathway to mature into the gp41 and gp120 subunits. Misfolded proteins located within the …
pathway to mature into the gp41 and gp120 subunits. Misfolded proteins located within the …
Protein engineering strategies with potential applications for altering clinically relevant cellular pathways at the protein level
All diseases can be fundamentally viewed as the result of malfunctioning cellular pathways.
Protein engineering offers the potential to develop new tools that will allow these …
Protein engineering offers the potential to develop new tools that will allow these …