Protein characterization plays a role in two key aspects of structural proteomics. The first is
the quality assessment of the produced protein preparations. Obtaining well diffracting …

Dengue is a mosquito-borne viral hemorrhagic disease that is a major threat to human
health in tropical and subtropical regions. Here we report crystal structures of a peptide …

Crystallization remains a critical step in X-ray structure determination. Because it is not
generally possible to rationally predict crystallization conditions, commercial screens have …

The presence of pseudo-symmetry in a macromolecular crystal and its interplay with
twinning may lead to an incorrect space-group (SG) assignment. Moreover, if the pseudo …

In recent times, there has been a large increase in the number of protein structures
deposited in the Protein Data Bank. Structural genomics initiatives have contributed to this …

The Structural Proteomics In Europe (SPINE) programme is aimed at the development and
implementation of high-throughput technologies for the efficient structure determination of …

In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-
ranging uses in molecular biology. In this chapter we describe the protocols used in the …

Background The three trypanosomatids pathogenic to men, Trypanosoma cruzi,
Trypanosoma brucei and Leishmania major, are etiological agents of Chagas disease …

A simple semi-automated microseeding procedure for nanolitre crystallization experiments
is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon …

Structural biology studies typically require large quantities of pure, soluble protein. Currently
the most widely-used method for obtaining such protein involves the use of bioinformatics …