Volume electron microscopy
Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
A connectome and analysis of the adult Drosophila central brain
The neural circuits responsible for animal behavior remain largely unknown. We summarize
new methods and present the circuitry of a large fraction of the brain of the fruit fly …
new methods and present the circuitry of a large fraction of the brain of the fruit fly …
[HTML][HTML] ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER
Cellular versatility depends on accurate trafficking of diverse proteins to their organellar
destinations. For the secretory pathway (followed by approximately 30% of all proteins), the …
destinations. For the secretory pathway (followed by approximately 30% of all proteins), the …
Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures
Abstract 3D correlative microscopy methods have revolutionized biomedical research,
allowing the acquisition of multidimensional information to gain an in-depth understanding …
allowing the acquisition of multidimensional information to gain an in-depth understanding …
A complete electron microscopy volume of the brain of adult Drosophila melanogaster
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-
neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only …
neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only …
The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigens
Abstract Type 1 conventional dendritic (cDC1) cells are necessary for cross-presentation of
many viral and tumor antigens to CD8+ T cells. cDC1 cells can be identified in mice and …
many viral and tumor antigens to CD8+ T cells. cDC1 cells can be identified in mice and …
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)
HGJ Damstra, B Mohar, M Eddison, A Akhmanova… - Elife, 2022 - elifesciences.org
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …
Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal
LE Newman, S Weiser Novak, GR Rojas… - Nature Cell …, 2024 - nature.com
Mitochondrial DNA (mtDNA) encodes essential subunits of the oxidative phosphorylation
system, but is also a major damage-associated molecular pattern (DAMP) that engages …
system, but is also a major damage-associated molecular pattern (DAMP) that engages …
A Connectome of the Adult Drosophila Central Brain
The neural circuits responsible for behavior remain largely unknown. Previous efforts have
reconstructed the complete circuits of small animals, with hundreds of neurons, and selected …
reconstructed the complete circuits of small animals, with hundreds of neurons, and selected …
Whole-brain serial-section electron microscopy in larval zebrafish
High-resolution serial-section electron microscopy (ssEM) makes it possible to investigate
the dense meshwork of axons, dendrites, and synapses that form neuronal circuits …
the dense meshwork of axons, dendrites, and synapses that form neuronal circuits …