This review discusses the challenges involved in the characterization of the glycosylation of
therapeutic glycoproteins. The focus is on methods that are most commonly used in …

The proper glycosylation of erythropoietin is essential for its function in vivo. Human
erythropoietins were isolated from Chinese hamster ovary cells transfected with a human …

Various deglycosylated derivatives of recombinant human erythropoietin (hEPO) were
prepared and used to determine the role of the sugar chains in the expression of its …

Revised Manuscript Received May 31, 1988 abstract: We have previously determined
thecarbohydrate structure of human recombinant erythropoietin [Sasaki, H „Bothner, B., Dell …

Human milk contains substantial concentrations of erythropoietin, a hormone best known for
its role in the regulation of erythropoiesis. Recent studies show that erythropoietin receptors …

IS generally accepted that in mammals under hypoxic conditions an ervthropoiesis
stimulating substance is produced. This substance, called the humoral erythropoietic factor …

Various partially or fully desialylated human erythropoietins were obtained by
neuraminidase digestion of the hormone, without non‐specific proteolysis and degradation …

ERYTHROPOIETIC activity in the plasma of rabbits made anæmic with phenylhydrazine is
associated with mucoproteins1–4. It has been shown that the activity is destroyed by …

A rapid quantitative analysis of the sialylated N-linked oligosaccharides of recombinant
erythropoietin (EPO) expressed in Chinese hamster ovary (CHO) cells has been developed …

Sera obtained from rabbits after immunization with human urinary ESF can neutralize the
biological activity of human urinary, sheep, rat, and rabbit plasma ESF. Such sera can …