Transcriptional control of ribosome biogenesis in yeast: links to growth and stress signals

D Shore, S Zencir, B Albert - Biochemical Society Transactions, 2021 - portlandpress.com
Ribosome biogenesis requires prodigious transcriptional output in rapidly growing yeast
cells and is highly regulated in response to both growth and stress signals. This minireview …

ChEC-seq2: an improved chromatin endogenous cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein–DNA interactions

J VanBelzen, C Duan, DG Brickner… - NAR Genomics and …, 2024 - academic.oup.com
Defining the in vivo DNA binding specificity of transcription factors (TFs) has relied nearly
exclusively on chromatin immunoprecipitation (ChIP). While ChIP reveals TF binding …

In situ tools for chromatin structural epigenomics

S Henikoff, K Ahmad - Protein Science, 2022 - Wiley Online Library
Technological progress over the past 15 years has fueled an explosion in genome‐wide
chromatin profiling tools that take advantage of low‐cost short‐read sequencing …

Chromatin endogenous cleavage provides a global view of yeast RNA polymerase II transcription kinetics

J VanBelzen, B Sakelaris, DG Brickner, N Marcou… - eLife, 2024 - elifesciences.org
Chromatin immunoprecipitation (ChIP-seq) is the most common approach to observe global
binding of proteins to DNA in vivo. The occupancy of transcription factors (TFs) from ChIP …

An improved ChEC-seq method accurately maps the genome-wide binding of transcription coactivators and sequence-specific transcription factors

R Donczew, A Lalou, D Devys, L Tora, S Hahn - biorxiv, 2021 - biorxiv.org
Mittal and colleagues have raised questions about mapping transcription factor locations on
DNA using the MNase-based ChEC-seq method. Partly due to this concern, we modified the …