Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

SF Hammad, IA Abdallah, A Bedair… - Journal of separation …, 2022 - Wiley Online Library
Liquid‐liquid extraction is a widely used technique of sample preparation in biomedical
analysis. In spite of the high pre‐concentration capacities of liquid–liquid extraction, it suffers …

Review of recent applications and modifications of aqueous two-phase system for the separation of biomolecules

A Segaran, LS Chua - International Journal of Biological Macromolecules, 2024 - Elsevier
Biomolecules, specifically proteins, polysaccharides, and secondary metabolites are
potential lead compounds due to their remarkable pharmacological properties. However, the …

Exploring the potential of all-aqueous immiscible systems for preparing complex biomaterials and cellular constructs

RC Gonçalves, MB Oliveira, JF Mano - Materials Horizons, 2024 - pubs.rsc.org
All-aqueous immiscible systems derived from liquid–liquid phase separation of incompatible
hydrophilic agents such as polymers and salts have found increasing interest in the …

Characterization and optimization of polymer-polymer aqueous two-phase systems for the isolation and purification of CaCo2 cell-derived exosomes

A Torres-Bautista, MA Torres-Acosta… - Plos one, 2022 - journals.plos.org
Exosomes are cell-derived vesicles that present attractive characteristics such as nano size
and unique structure for their use as drug delivery systems for drug therapy, biomarkers for …

Thermosensitive polymer‐assisted extraction and purification of fungal laccase from citrus pulp wash effluent

CEM Santana, GP Barros, NS Canuto… - Journal of the …, 2024 - Wiley Online Library
BACKGROUND This study explores the use of liquid–liquid extraction with thermosensitive
polymers for producing laccase (Lac) from Pleurotus sajor‐caju. This process leverages …

[HTML][HTML] Reactive aqueous two-phase systems for the production and purification of PEGylated proteins

VR Campos-García, J Benavides… - Electronic Journal of …, 2021 - Elsevier
Background PEGylation, defined as the covalent attachment of polyethylene glycol, allows
the synthesis of PEGylated therapeutic proteins with enhanced physicochemical properties …

Liquid–Liquid–Liquid Aqueous Triple-Phase System Based on DES and Dipotassium Hydrogen Phosphate

J Fu, X Wu, Q Xu, Y Wang, Y Yang… - Journal of Chemical & …, 2024 - ACS Publications
Deep eutectic solvent (DES) and inorganic salt can form a salt–salt aqueous two-phase
system (ATPS) and have been widely studied recently. The DES-based ATPS extends the …

A monolithic stationary phase with dendritic nanostructures for the separation of PEGylated proteins

BE De los Santos‐González… - …, 2023 - Wiley Online Library
Separation of PEGylated protein mixtures into individual species is a challenging procedure,
and many efforts have been focused on creating novel chromatographic supports for this …

New methodologies and equipment used in new-generation separation and preconcentration methods

MHA Azqhandi, T Khezeli, M Ghaedi… - New Generation Green …, 2020 - Elsevier
Separation, purification, and preconcentration of different components from their
complicated matrices by immiscible solvents and/or different sources of the receiving phase …

Advances, current challenges, and future trends in bioseparation: perspective analysis of the papers published in JCTB

M González‐González… - Journal of Chemical …, 2022 - Wiley Online Library
Bioseparation is a crucial process for the obtention of biological products that are essential
in daily life, such as medicine and food. At first, the researchers centered their efforts on the …