Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …

The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …

The production efficiency of microbial cell factories is sometimes limited by the lack of
effective methods to regulate multiple targets in a coordinated manner. Here taking the …

Synthetic biology toolkits are one of the core foundations on which the field has been built,
facilitating and accelerating efforts to reprogram cells and organisms for diverse …

Plant morphology and anatomy strongly influence agricultural yield. Crop domestication has
strived for desirable growth and developmental traits, such as larger and more fruits and …

The CRISPR-Cas systems have shown tremendous promise as heterologous tools for
genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and …

Corynebacterium glutamicum is a promising organism for the industrial production of amino
acids, fuels, and various value-added chemicals. From the whole genome sequence …

Microbial catalysts must partition incoming substrate between the synthesis of biomass and
the synthesis of a desired product. Although biomass synthesis generates more catalyst and …

The CRISPR-Cas system has enabled the development of sophisticated, multigene
metabolic engineering programs through the use of guide RNA-directed activation or …

Chinese hamster ovary (CHO) cells are among the most widely used mammalian cell lines
in the biopharmaceutical industry. Therefore, it is not surprising that significant efforts have …