Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …

The human gene catalogue is essentially complete, but we lack an equivalently vetted
inventory of bona fide human enhancers. Hundreds of thousands of candidate enhancers …

Many chromatin features play critical roles in regulating gene expression. A complete
understanding of gene regulation will require the mapping of specific chromatin features in …

Identifying the transcription factors (TFs) responsible for observed changes in gene
expression is an important step in understanding gene regulatory networks. ChIP-X …

Cohesin folds mammalian interphase chromosomes by extruding the chromatin fiber into
numerous loops.“Loop extrusion” can be impeded by chromatin-bound factors, such as …

Cleavage under targets and release using nuclease (CUT&RUN) is an epigenomic profiling
strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases …

Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN,
in which unfixed permeabilized cells are incubated with antibody, followed by binding of a …

Whereas folding of genomes at the large scale of epigenomic compartments and
topologically associating domains (TADs) is now relatively well understood, how chromatin …

We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a
chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal …

Background CUT&RUN is an efficient epigenome profiling method that identifies sites of
DNA binding protein enrichment genome-wide with high signal to noise and low sequencing …