Trends in sample preparation for classical and second generation proteomics

B Cañas, C Piñeiro, E Calvo, D López-Ferrer… - … of chromatography A, 2007 - Elsevier
Sample preparation is a fundamental step in the proteomics workflow. However, it is not
easy to find compiled information updating this subject. In this paper, the strategies and …

Recent advances in mass spectrometry (MS)-based glycoproteomics in complex biological samples

Z Chen, J Huang, L Li - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Protein glycosylation plays a key role in various biological processes and disease-related
pathological progression. Mass spectrometry (MS)-based glycoproteomics is a powerful …

The CoQH2/CoQ ratio serves as a sensor of respiratory chain efficiency

A Guaras, E Perales-Clemente, E Calvo, R Acín-Pérez… - Cell reports, 2016 - cell.com
Electrons feed into the mitochondrial electron transport chain (mETC) from NAD-or FAD-
dependent enzymes. A shift from glucose to fatty acids increases electron flux through FAD …

General statistical framework for quantitative proteomics by stable isotope labeling

P Navarro, M Trevisan-Herraz… - Journal of proteome …, 2014 - ACS Publications
The combination of stable isotope labeling (SIL) with mass spectrometry (MS) allows
comparison of the abundance of thousands of proteins in complex mixtures. However …

Defective sarcoplasmic reticulum–mitochondria calcium exchange in aged mouse myocardium

C Fernandez-Sanz, M Ruiz-Meana, E Miro-Casas… - Cell Death & …, 2014 - nature.com
Mitochondrial alterations are critically involved in increased vulnerability to disease during
aging. We investigated the contribution of mitochondria–sarcoplasmic reticulum (SR) …

18O Stable Isotope Labeling in MS-based Proteomics

X Ye, B Luke, T Andresson… - Briefings in functional …, 2009 - academic.oup.com
A variety of stable isotope labeling techniques have been developed and used in mass
spectrometry (MS)-based proteomics, primarily for relative quantitation of changes in protein …

A robust method for quantitative high-throughput analysis of proteomes by 18O labeling

E Bonzon-Kulichenko, D Perez-Hernandez… - Molecular & Cellular …, 2011 - ASBMB
MS-based quantitative proteomics plays an increasingly important role in biological and
medical research and the development of these techniques remains one of the most …

The use of bacteriophages in biotechnology and recent insights into proteomics

AG Abril, M Carrera, V Notario, Á Sánchez-Pérez… - Antibiotics, 2022 - mdpi.com
Phages have certain features, such as their ability to form protein–protein interactions, that
make them good candidates for use in a variety of beneficial applications, such as in human …

Proteome-wide alterations on adipose tissue from obese patients as age-, diabetes-and gender-specific hallmarks

M Gómez-Serrano, E Camafeita, E García-Santos… - Scientific reports, 2016 - nature.com
Obesity is a main global health issue and an outstanding cause of morbidity and mortality
predisposing to type 2 diabetes (T2DM) and cardiovascular diseases. Huge research efforts …

A novel strategy for global analysis of the dynamic thiol redox proteome

P Martínez-Acedo, E Nunez, FJS Gomez… - Molecular & Cellular …, 2012 - ASBMB
Nitroxidative stress in cells occurs mainly through the action of reactive nitrogen and oxygen
species (RNOS) on protein thiol groups. Reactive nitrogen and oxygen species-mediated …