In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-
resolution analysis of the structure and topology of transmembrane proteins in native-like …

We used oriented solid-state NMR spectroscopy and biochemical cross-linking experiments
to demonstrate that the ligand-free membrane protein transporter EmrE forms anti-parallel …

EmrE is a multidrug resistance efflux pump with specificity to a wide range of antibiotics and
antiseptics. To obtain atomic-scale insight into the attributes of the native state that encodes …

Phospholamban (PLN) is a mini-membrane protein that directly controls the cardiac Ca2+-
transport response to β-adrenergic stimulation, thus modulating cardiac output during the …

Oriented solid-state NMR is the most direct methodology to obtain the orientation of
membrane proteins with respect to the lipid bilayer. The method consists of measuring 1 H …

We present a new method called DAISY (Dual Acquisition orIented ssNMR spectroScopY)
for the simultaneous acquisition of 2D and 3D oriented solid-state NMR experiments for …

We present a strategy dubbed CURD (correlations using recycle delays) to acquire chemical-
shift assignments and distance restraints for proteins in a single experimental block under …

Pulse schemes with direct observation of multiple free induction decays (FIDs) offer a
dramatic increase in the spectral information content of NMR experiments and often yield …

Oriented sample solid-state NMR (OS-ssNMR) spectroscopy is uniquely suited to determine
membrane protein topology at the atomic resolution in liquid crystalline bilayers under …