Sample preparation is considered as the bottleneck step in bioanalysis because each
biological matrix has its own unique challenges and complexity. Competent sample …

The potential of antisense oligonucleotides in gene silencing was discovered over 40 years
ago, which resulted in the growing interest in their chemistry, mechanism of action, and …

Oligonucleotides (OGNs) are relatively new modalities that offer unique opportunities to
expand the therapeutic targets. Reliable and high-throughput bioanalytical methods are …

Oligonucleotides have many important applications, including as primers in polymerase
chain reactions and probes for DNA sequencing. They are proposed as a diagnostic and …

The present investigation showed that each of the three different liquid chromatography
modes may be successfully used for the qualitative analysis of nusinersen metabolites in a …

Drug properties of antisense oligonucleotides (ASOs) differ significantly from those of
traditional small-molecule therapeutics. In this review, we focus on ASO disposition, mainly …

Considerable advances have been made in the research and development of
oligonucleotide therapeutics (OTs) for treating central nervous system (CNS) diseases, such …

Recently, non-coding RNA (ncRNA) became the centerpiece of human genome research.
Modern ncRNA-based research has revolutionized disease diagnosis and therapeutics …

Mass spectrometry-based proteomics experiments can be subject to a large variability,
which forms an obstacle to obtaining deep and accurate protein identification. Here, to …

Abstract Aim: Quantitative LC–MS analysis of oligonucleotides (OGNs) in biological matrices
is needed to support candidate selection of new therapeutic OGNs. Methodology & results: A …