Two years into the COVID-19 pandemic: lessons learned

SJR da Silva, JCF do Nascimento… - ACS infectious …, 2022 - ACS Publications
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible
and virulent human-infecting coronavirus that emerged in late December 2019 in Wuhan …

Obtaining reliable RT-qPCR results in molecular diagnostics—MIQE goals and pitfalls for transcriptional biomarker discovery

C Grätz, MLU Bui, G Thaqi, B Kirchner, RP Loewe… - Life, 2022 - mdpi.com
In this review, we discuss the development pipeline for transcriptional biomarkers in
molecular diagnostics and stress the importance of a reliable gene transcript quantification …

Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

L Bokelmann, O Nickel, T Maricic, S Pääbo… - Nature …, 2021 - nature.com
Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and
cost-effective diagnostic methods which can be applied to large numbers of people …

Self-collected gargle lavage allows reliable detection of SARS-CoV-2 in an outpatient setting

J Zander, S Scholtes, M Ottinger, M Kremer… - Microbiology …, 2021 - Am Soc Microbiol
Current procurement of specimens for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) detection requires trained personnel and dedicated equipment. We …

Performance of saline and water gargling for SARS-CoV-2 reverse transcriptase PCR testing: a systematic review and meta-analysis

NNY Tsang, HC So, BJ Cowling… - European Respiratory …, 2022 - Eur Respiratory Soc
The performance of gargling for severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) reverse transcriptase (RT)-PCR testing has not been previously reviewed. This …

[HTML][HTML] Validación clínica de la prueba RT-LAMP para el diagnóstico rápido del SARS-CoV-2

L Hurtado, D Díaz, K Escorcia, L Flórez, Y Bello, Y Díaz… - Biomédica, 2022 - scielo.org.co
Introducción. Desde el primer reporte en la provincia de Wuhan (China) en el año 2019, el
SARS-CoV-2 se ha diseminado por todo el mundo, provocando un enorme impacto en la …

Extraction-free clinical detection of SARS-CoV-2 virus from saline gargle samples using Hamilton STARlet liquid handler

VJ Gadkar, DM Goldfarb, GN Al-Rawahi, JA Srigley… - Scientific Reports, 2023 - nature.com
As part of the COVID-19 pandemic, clinical laboratories have been faced with massive
increases in testing, resulting in sample collection systems, reagent, and staff shortages. We …

Usefulness of rapid antigen testing for SARS-CoV-2 screening of healthcare workers: a pilot study

A Šterbenc, V Tomič, U Bidovec Stojković… - Clinical and …, 2022 - Springer
Background Identification of infected healthcare workers (HCWs) is an important step in
severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) transmission control. Rapid …

[PDF][PDF] Strategies for scaling up SARS-CoV-2 molecular testing capacity

S Das, KM Frank - Clinics in laboratory medicine, 2022 - Elsevier
More than 413 million people have been infected with the severe acute respiratory
syndrome coronavirus-2 (SARS-CoV-2) resulting in more than 5.8 million deaths worldwide …

The Comparative Analysis of Two RT‐qPCR Kits for Detecting SARS‐CoV‐2 Reveals a Higher Risk of False‐Negative Diagnosis in Samples with High Quantification …

R Luraschi, C Barrera-Avalos… - Canadian Journal of …, 2022 - Wiley Online Library
The early detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)
using the real‐time quantitative polymerase chain reaction (RT‐qPCR) as a gold‐standard …