Artificial cells: past, present and future

W Jiang, Z Wu, Z Gao, M Wan, M Zhou, C Mao… - ACS nano, 2022 - ACS Publications
Artificial cells are constructed to imitate natural cells and allow researchers to explore
biological process and the origin of life. The construction methods for artificial cells, through …

[HTML][HTML] Coacervates as models of membraneless organelles

NA Yewdall, AAM André, T Lu, E Spruijt - Current Opinion in Colloid & …, 2021 - Elsevier
Coacervates are condensed liquid-like droplets, usually formed with oppositely charged
polymeric molecules. They have been studied extensively in colloid and interface science …

Protocells: Milestones and recent advances

I Gözen, ES Köksal, I Põldsalu, L Xue, K Spustova… - Small, 2022 - Wiley Online Library
The origin of life is still one of humankind's great mysteries. At the transition between
nonliving and living matter, protocells, initially featureless aggregates of abiotic matter, gain …

Membrane-confined liquid-liquid phase separation toward artificial organelles

W Mu, Z Ji, M Zhou, J Wu, Y Lin, Y Qiao - Science advances, 2021 - science.org
As the basic unit of life, cells are compartmentalized microreactors with molecularly crowded
microenvironments. The quest to understand the cell origin inspires the design of synthetic …

Recent advances in droplet microfluidics

Y Ding, PD Howes, AJ deMello - Analytical chemistry, 2019 - ACS Publications
Prominent within this technology set are droplet-based microfluidic systems, 3 in which
passive microfluidic structures are used to rapidly generate and manipulate subnanoliter …

Enzyme-mediated nitric oxide production in vasoactive erythrocyte membrane-enclosed coacervate protocells

S Liu, Y Zhang, M Li, L Xiong, Z Zhang, X Yang… - Nature Chemistry, 2020 - nature.com
The design and construction of synthetic therapeutic protocells capable of establishing
cognate chemical communication channels with living cells is an important challenge for …

Endocytosis of coacervates into liposomes

T Lu, S Liese, L Schoenmakers, CA Weber… - Journal of the …, 2022 - ACS Publications
Recent studies have shown that the interactions between condensates and biological
membranes are of functional importance. Here, we study how the interaction between …

Giant coacervate vesicles as an integrated approach to cytomimetic modeling

Y Zhang, Y Chen, X Yang, X He, M Li… - Journal of the …, 2021 - ACS Publications
Although giant unilamellar vesicles (GUVs) have been extensively studied as synthetic cell-
like microcompartments, their applicability as cytomimetic models is severely compromised …

Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates

RR Poudyal, RM Guth-Metzler, AJ Veenis… - Nature …, 2019 - nature.com
Membraneless compartments, such as complex coacervates, have been hypothesized as
plausible prebiotic micro-compartments due to their ability to sequester RNA; however, their …

Development and future of droplet microfluidics

L Nan, H Zhang, DA Weitz, HC Shum - Lab on a Chip, 2024 - pubs.rsc.org
Over the past two decades, advances in droplet-based microfluidics have facilitated new
approaches to process and analyze samples with unprecedented levels of precision and …