Real-time on-site diagnosis of quarantine pathogens in plant tissues by nanopore-based sequencing

L Marcolungo, A Passera, S Maestri, E Segala… - Pathogens, 2022 - mdpi.com
Rapid and sensitive assays for the identification of plant pathogens are necessary for the
effective management of crop diseases. The main limitation of current diagnostic testing is …

Reference-free plant disease detection using machine learning and long-read metagenomic sequencing

MA Johnson, BA Vinatzer, S Li - Applied and Environmental …, 2023 - Am Soc Microbiol
Surveillance for early disease detection is crucial to reduce the threat of plant diseases to
food security. Metagenomic sequencing and taxonomic classification have recently been …

Investigating plant disease outbreaks with long-read metagenomics: sensitive detection and highly resolved phylogenetic reconstruction applied to Xylella fastidiosa

MA Johnson, H Liu, E Bush, P Sharma… - Microbial …, 2022 - microbiologyresearch.org
Early disease detection is a prerequisite for enacting effective interventions for disease
control. Strains of the bacterial plant pathogen Xylella fastidiosa have recurrently spread to …

Century-old herbarium specimen provides insights into Pierce's disease of grapevines emergence in the Americas

MA Donegan, AK Kahn, N Becker, AC Siri, PE Campos… - Current Biology, 2025 - cell.com
Fossils and other preserved specimens are integral for informing timing and evolutionary
history in every biological system. By isolating a plant pathogen genome from herbarium …

Concept note: Toward metagenomic sequencing for rapid, sensitive, and accurate detection of bacterial pathogens in potato seed production

V Roman-Reyna, R Rioux, B Babler, T Klass… - PhytoFrontiers …, 2023 - Am Phytopath Society
Disease-free planting stocks and formal certification systems are critical for the profitable
production of vegetatively propagated crops. Propagation through seed tubers is the …

Live tracking of a plant pathogen outbreak reveals rapid and successive, multidecade plasmid reduction

V Roman-Reyna, A Sharma, H Toth, Z Konkel… - Msystems, 2024 - Am Soc Microbiol
Quickly understanding the genomic changes that lead to pathogen emergence is necessary
to launch mitigation efforts and reduce harm. In this study, we tracked in real time a 2022 …

A Survey of Xylella fastidiosa in the US State of Virginia Reveals Wide Distribution of Both Subspecies fastidiosa and multiplex in Grapevine

S Abdelrazek, E Bush, C Oliver, H Liu… - …, 2024 - Am Phytopath Society
Global travel and trade in combination with climate change are expanding the geographic
distribution of plant pathogens. The bacterium Xylella fastidiosa is a prime example. Native …

Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR

J Li, R Chen, R Yang, X Wei, H Xie, Y Shi, X Xie… - Microorganisms, 2023 - mdpi.com
Pectobacterium brasiliense (Pbr) has caused significant economic losses in major vegetable
production areas in Northern China by causing bacterial soft rot in cash crops such as …

Draft Genome Sequence Resource of Xylella fastidiosa Strain Alm_Lz_1 Associated with a New Outbreak in Lazio, Italy

A Giampetruzzi, G Loconsole, S Zicca… - …, 2023 - Am Phytopath Society
In November 2021, in the framework of the annual surveillance program implemented in all
European Union countries, an outbreak of Xylella fastidiosa was intercepted in the Italian …

Live tracking of a plant pathogen outbreak reveals rapid and successive, multidecade episome reduction

V Roman-Reyna, A Sharma, H Toth, Z Konkel… - bioRxiv, 2023 - biorxiv.org
Quickly understanding the genomic changes that lead to pathogen emergence is necessary
to launch mitigation efforts and reduce harm. Often the evolutionary events that result in an …