Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae

S He, Z Zhang, W Lu - Journal of Industrial Microbiology and …, 2023 - academic.oup.com
Sharomyces cerevisiae is currently one of the most important foreign gene expression
systems. S. cerevisiae is an excellent host for high-value metabolite cell factories due to its …

The evolution of the GALactose utilization pathway in budding yeasts

MC Harrison, AL LaBella, CT Hittinger, A Rokas - Trends in Genetics, 2022 - cell.com
The Leloir galactose utilization or GAL pathway of budding yeasts, including that of the
baker's yeast Saccharomyces cerevisiae and the opportunistic human pathogen Candida …

SOX9 switch links regeneration to fibrosis at the single-cell level in mammalian kidneys

S Aggarwal, Z Wang, D Rincon Fernandez Pacheco… - Science, 2024 - science.org
The steps governing healing with or without fibrosis within the same microenvironment are
unclear. After acute kidney injury (AKI), injured proximal tubular epithelial cells activate …

Transcription factor clusters enable target search but do not contribute to target gene activation

JVW Meeussen, W Pomp, I Brouwer… - Nucleic Acids …, 2023 - academic.oup.com
Many transcription factors (TFs) localize in nuclear clusters of locally increased
concentrations, but how TF clustering is regulated and how it influences gene expression is …

Machine learning enables identification of an alternative yeast galactose utilization pathway

MC Harrison, EJ Ubbelohde… - Proceedings of the …, 2024 - National Acad Sciences
How genomic differences contribute to phenotypic differences is a major question in biology.
The recently characterized genomes, isolation environments, and qualitative patterns of …

A suite of constitutive promoters for tuning gene expression in plants

A Zhou, LD Kirkpatrick, IJ Ornelas… - ACS Synthetic …, 2023 - ACS Publications
The need for convenient tools to express transgenes over a large dynamic range is
pervasive throughout plant synthetic biology; however, current efforts are largely limited by …

Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production

Y Zhang, M Su, Y Chen, Z Wang, J Nielsen… - Biotechnology for Biofuels …, 2023 - Springer
Background With unique physiochemical environments in subcellular organelles, there has
been growing interest in harnessing yeast organelles for bioproduct synthesis. Among these …

The trans-regulatory landscape of gene networks in plants

NFC Hummel, A Zhou, B Li, K Markel, IJ Ornelas… - Cell Systems, 2023 - cell.com
The transcriptional effector domains of transcription factors play a key role in controlling
gene expression; however, their functional nature is poorly understood, hampering our …

[HTML][HTML] Cell-cycle dependent nuclear gene delivery enhances the effects of E-cadherin against tumor invasion and metastasis

L Xie, J Wang, L Song, T Jiang, F Yan - Signal Transduction and …, 2023 - nature.com
Gene delivery is the process by which foreign DNA is transferred to host cells, released from
intracellular vesicles, and transported to the nuclei for transcription. This process is …

Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae

X Zhao, H Yu, Q Liang, J Zhou, J Li… - Journal of Agricultural …, 2023 - ACS Publications
Horseradish peroxidase (HRP) is a plant-derived glycoprotein that can be developed as a
food additive to cross-link proteins or biopolymers. Although Saccharomyces cerevisiae has …