Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …
dramatically expanded in recent years, both in terms of applications and widespread …
A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines
Cell-free protein synthesis systems that can be lyophilized for long-term, non-refrigerated
storage and transportation have the potential to enable decentralized biomanufacturing …
storage and transportation have the potential to enable decentralized biomanufacturing …
Constructing cell-free expression systems for low-cost access
Cell-free systems for gene expression have gained attention as platforms for the facile study
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane …
attention for biomanufacturing and synthetic biology. However, activating membrane …
Rapid RNase inhibitor production to enable low‐cost, on‐demand cell‐free protein synthesis biosensor use in human body fluids
Human body fluids contain biomarkers which are used extensively for prognostication,
diagnosis, monitoring, and evaluation of different treatments for a variety of diseases and …
diagnosis, monitoring, and evaluation of different treatments for a variety of diseases and …
The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare
The cell-free system is a tool that harnesses the complex biological processes outside of
living cells. Owing to the system's superior flexibility and manipulability, it has matured its …
living cells. Owing to the system's superior flexibility and manipulability, it has matured its …
Characterizing and Improving Reaction Times for E. coli-Based Cell-Free Protein Synthesis
LR Burrington, KR Watts, JP Oza - ACS Synthetic Biology, 2021 - ACS Publications
Cell-free protein synthesis (CFPS) is a platform biotechnology that has enabled the on-
demand synthesis of proteins for a variety of applications. Numerous advances have …
demand synthesis of proteins for a variety of applications. Numerous advances have …
Rapid sensing of clinically relevant glutamine concentrations in human serum with metabolically engineered E. coli-based cell-free protein synthesis
JP Hunt, RJ Barnett, H Robinson, M Soltani… - Journal of …, 2021 - Elsevier
Bioavailable glutamine (Gln) is critical for metabolism, intestinal health, immune function,
and cell signaling. Routine measurement of serum Gln concentrations could facilitate …
and cell signaling. Routine measurement of serum Gln concentrations could facilitate …
Characterizing and improving pET vectors for cell-free expression
K Jew, PEJ Smith, B So, J Kasman, JP Oza… - … in Bioengineering and …, 2022 - frontiersin.org
Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …