Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) systems provide adaptive immunity against viruses and plasmids in bacteria and …

Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …

Clustered regularly interspaced short palindromic repeat (CRISPR) is a recently discovered
adaptive prokaryotic immune system that provides acquired immunity against foreign nucleic …

This volume is a timely and comprehensive description of the many facets of DNA and RNA
modification-editing processes and to some extent repair mechanisms. Each chapter offers …

A 2-aminothieno [3, 4-d] pyrimidine G-mimic deoxyribonucleoside, thdG, was synthesized
and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue …

DNA cytosine methylation is a widespread epigenetic mark. Biological effects of DNA
methylation are mediated by the proteins that preferentially bind to 5-methylcytosine (5mC) …

Probe molecules that enable the detection of specific DNA sequences are used in
diagnostic and basic research. Most methods rely on the specificity of hybridization …

Quadruplexes are involved in the regulation of gene expression and are part of telomeres at
the ends of chromosomes. In addition, they are useful in therapeutic and biotechnological …

In the process of transcription initiation, the bacterial RNA polymerase binds double-
stranded (ds) promoter DNA and subsequently effects strand separation of 12 to 14 base …

EcoRII restriction endonuclease is specific for the 5′-CCWGG sequence (W stands for A or
T); however, it shows no activity on a single recognition site. To activate cleavage it requires …