Amplification-free nucleic acids detection with next-generation CRISPR/dx systems
CH Lau, S Huang, H Zhu - Critical Reviews in Biotechnology, 2024 - Taylor & Francis
CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular
diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT) …
diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT) …
Fluorescent nucleic acid probes for DNA repair enzymes: Design strategies and applications
X Luan, X Tang, J Deng, Y Yang, J Zhai… - TrAC Trends in Analytical …, 2024 - Elsevier
DNA repair enzymes play a vital role in the maintenance of genomic integrity, and the
development of multiple diseases. Investigation of DNA repair enzymes is of great …
development of multiple diseases. Investigation of DNA repair enzymes is of great …
Ultrasensitive turn-on detection of biomarker FEN1 using a CRISPR/Cas13a system integrated with a cleavage-ligation-activation cascade
Q Han, X Bian, Y Chen, B Li - Sensors and Actuators B: Chemical, 2023 - Elsevier
Flap endonuclease I (FEN1) is a structure-specific nuclease that participates in DNA repair,
and its overexpression is related to an increased risk of cancer. Currently, few FEN1 assays …
and its overexpression is related to an increased risk of cancer. Currently, few FEN1 assays …
CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level
N Zhao, X Tian, F Ma, C Zhang - Chemical Communications, 2023 - pubs.rsc.org
We develop a new fluorescent biosensor for flap endonuclease 1 (FEN1) assay based on
CRISPR/Cas12-enhanced single-molecule counting. This biosensor is simple, selective …
CRISPR/Cas12-enhanced single-molecule counting. This biosensor is simple, selective …
Self-Powered FEN1 Biosensor Based on Accelerated CRISPR/Cas Trans-Cleavage around Porous Fe3O4 Nanoparticles
Y Jiang, X Chen, P Miao, N Feng - ACS Applied Materials & …, 2024 - ACS Publications
Flap endonuclease 1 (FEN1) is a structure-specific endonuclease that plays a critical role in
the maintenance of genome integrity. In this work, we demonstrate a novel self-powered …
the maintenance of genome integrity. In this work, we demonstrate a novel self-powered …
Microfluidic particle counter visualizing mucosal antibodies against SARS-CoV-2 in the upper respiratory tract for rapid evaluation of immune protection
Mucosal antibodies in the upper respiratory tract are the earliest and most critical
responders to prevent respiratory infections, providing an indication for the rapid evaluation …
responders to prevent respiratory infections, providing an indication for the rapid evaluation …
An ultrasensitive biosensor with suppressed background signals for FEN1 detection in a homogeneous reaction via cascade primer exchange reaction and CRISPR …
YL Song, XL He, MM Pan, M Wang, M Jiang… - Sensors and Actuators B …, 2024 - Elsevier
Abstract Flap endonuclease 1 (FEN1) is a structure-specific enzyme which plays a crucial
role in DNA replication and repair within mammalian cells. FEN1 is also known to be …
role in DNA replication and repair within mammalian cells. FEN1 is also known to be …
Dual signal amplification-integrated single-molecule biosensing of flap endonuclease 1 in breast cancer tissues
F Ma, W Liu, H Liu, Q Xu, C Zhang - Sensors and Actuators B: Chemical, 2023 - Elsevier
Abstract Flap endonuclease 1 (FEN1) is a structure-specific nuclease that may act as a
valuable biomarker for cancer diagnosis. Herein, we construct a dual signal amplification …
valuable biomarker for cancer diagnosis. Herein, we construct a dual signal amplification …
Homogeneous detection of viral nucleic acid via selective recognition proximity ligation and signal amplification with T7 transcription and CRISPR/Cas12a system
YL Song, XL He, Y Li, M Wang, M Jiang, L Xu… - Analytica Chimica Acta, 2023 - Elsevier
The synthetic biology has employed the synthetic gene networks through engineering to
construct various functions in biological systems. However, the use of gene circuits to create …
construct various functions in biological systems. However, the use of gene circuits to create …
An ultra-sensitive fluorescence biosensor with rolling circle amplification and CRISPR/Cas12a one-pot system for FEN1 detection
XL He, YL Song, LY Ma, M Jiang, L Xu, X Yu - Talanta, 2025 - Elsevier
Abstract Flap endonuclease 1 (FEN1), a structure-specific nuclease, has been reported to
be widely involved in the development of cancer, and recognized as a new biomarker for …
be widely involved in the development of cancer, and recognized as a new biomarker for …