Directed evolution has revolutionized biomolecular engineering by applying cycles of
mutation, amplification and selection to genes of interest (GOIs). However, classical directed …

Alteration in the target sites of antibiotics is a common mechanism of resistance. Examples
of clinical strains showing resistance can be found for every class of antibiotic, regardless of …

Directed evolution is a powerful approach for engineering biomolecules and understanding
adaptation. However, experimental strategies for directed evolution are notoriously labor …

Due to their remarkable parasitocidal activity, artemisinins represent the key components of
first-line therapies against Plasmodium falciparum malaria. However, the decline in efficacy …

Increasing resistance of Plasmodium falciparum malaria parasites to chloroquine and the
dihydrofolate reductase (DHFR) inhibitors pyrimethamine and cycloguanil have sparked …

Malarial dihydrofolate reductase (DHFR) is the target of antifolate antimalarial drugs such as
pyrimethamine and cycloguanil, the clinical efficacy of which have been compromised by …

Plasmodium falciparum dihydrofolate reductase–thymidylate synthase (PfDHFR-TS) is an
important target of antimalarial drugs. The efficacy of this class of DHFR-inhibitor drugs is …

Chemotherapy remains the only practicable tool to control falciparum malaria in sub-
Saharan Africa, where> 90% of the world's burden of malaria mortality and morbidity occurs …

To assess the relationship between mutations in Plasmodium falciparum dihydrofolate
reductase (DHFR) and dihydropteroate synthase (DHPS) and clinical pyrimethamine …

Since ancient times, humankind has had to struggle against the persistent onslaught of
pathogenic microorganisms. Nowadays, malaria is still the most important infectious disease …