[HTML][HTML] Live-cell imaging in the deep learning era

JW Pylvänäinen, E Gómez-de-Mariscal… - Current Opinion in Cell …, 2023 - Elsevier
Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Focal adhesion dynamics in cellular function and disease

YG Mishra, B Manavathi - Cellular signalling, 2021 - Elsevier
Acting as a bridge between the cytoskeleton of the cell and the extra cellular matrix (ECM),
the cell-ECM adhesions with integrins at their core, play a major role in cell signalling to …

Democratising deep learning for microscopy with ZeroCostDL4Mic

L von Chamier, RF Laine, J Jukkala, C Spahn… - Nature …, 2021 - nature.com
Deep Learning (DL) methods are powerful analytical tools for microscopy and can
outperform conventional image processing pipelines. Despite the enthusiasm and …

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

V Louvel, R Haase, O Mercey, MH Laporte… - Nature …, 2023 - nature.com
Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …

Practical considerations for quantitative light sheet fluorescence microscopy

CM Hobson, M Guo, HD Vishwasrao, Y Wu, H Shroff… - Nature …, 2022 - nature.com
Fluorescence microscopy has evolved from a purely observational tool to a platform for
quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic …

Unravelling cell migration: defining movement from the cell surface

F Merino-Casallo, MJ Gomez-Benito… - Cell adhesion & …, 2022 - Taylor & Francis
Cell motility is essential for life and development. Unfortunately, cell migration is also linked
to several pathological processes, such as cancer metastasis. Cells' ability to migrate relies …

Quantification of tumor heterogeneity: from data acquisition to metric generation

A Kashyap, MA Rapsomaniki, V Barros… - Trends in …, 2022 - cell.com
Tumors are unique and complex ecosystems, in which heterogeneous cell subpopulations
with variable molecular profiles, aggressiveness, and proliferation potential coexist and …

Bright and stable monomeric green fluorescent protein derived from StayGold

H Zhang, GD Lesnov, OM Subach, W Zhang… - Nature …, 2024 - nature.com
The high brightness and photostability of the green fluorescent protein StayGold make it a
particularly attractive probe for long-term live-cell imaging; however, its dimeric nature …

Presynapses contain distinct actin nanostructures

D Bingham, CE Jakobs, F Wernert… - Journal of Cell …, 2023 - rupress.org
The architecture of the actin cytoskeleton that concentrates at presynapses remains poorly
known, hindering our understanding of its roles in synaptic physiology. In this work, we …

Extending resolution within a single imaging frame

E Torres-García, R Pinto-Cámara, A Linares… - Nature …, 2022 - nature.com
The resolution of fluorescence microscopy images is limited by the physical properties of
light. In the last decade, numerous super-resolution microscopy (SRM) approaches have …