Highlights•NCp7 plays several roles in the viral life cycle.•Covalent NCp7 inhibitors are able
to extrude the zinc from the protein.•The SAR of covalent NCp7 inhibitors is discussed.•The …

The duality of liquid-liquid phase separation (LLPS) of cellular components into
membraneless organelles defines the nucleation of both normal and disease processes …

Metabolic reactions in living cells are limited by diffusion of reagents in the cytoplasm. Any
attempt to quantify the kinetics of biochemical reactions in the cytosol should be preceded by …

Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on
measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes …

This work, based on in vivo and in vitro measurements, as well as in silico simulations,
provides a consistent analysis of diffusion of polydisperse nanoparticles in the cytoplasm of …

Raster image correlation spectroscopy (RICS) is a powerful method for measuring molecular
diffusion in live cells directly from images acquired on a laser scanning microscope …

The human immunodeficiency virus-1 (HIV-1) nucleocapsid protein (NCp7) is a nucleic acid
chaperone protein with two highly conserved zinc fingers. To exert its key roles in the viral …

Arc, also known as Arg3. 1, is an activity-dependent immediate–early gene product that
plays essential roles in memory consolidation. A pool of Arc is located in the postsynaptic …

Biochemistry in living cells is an emerging field of science. Current quantitative bioassays
are performed ex vivo, thus equilibrium constants and reaction rates of reactions occurring in …

The human immunodeficiency virus type 1 Gag precursor specifically selects the unspliced
viral genomic RNA (gRNA) from the bulk of cellular and spliced viral RNAs via its …