Synthetic biology has mainly focused on introducing new or altered functionality in single
cell systems: primarily bacteria, yeast, or mammalian cells. Here, we describe the extension …

Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a mo dular s afe-harbor t ransgene i nsertion (MosTI) …

Myosin storage myopathy (MSM) is a rare skeletal muscle disorder caused by mutations in
the slow muscle/β-cardiac myosin heavy chain (MHC) gene. MSM missense mutations …

The ability to manipulate gene activity and control transgene expression is essential to study
gene function. While several genetic tools for modifying genes or controlling expression …

A core tenet of synthetic biology is that well-characterized regulatory elements are essential
for engineering biological systems. Here, we characterize the specificity and expression …

Chloride intracellular channels (CLICs) are conserved proteins for which the cellular and
molecular functions remain mysterious. An important insight into CLIC function came from …

To understand the function of cells such as neurons within an organism, it can be
instrumental to inhibit cellular function, or to remove the cell (type) from the organism, and …

Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify
cell-specific promoters for every class of neuron in C. elegans. Here, we characterize a short …

The apical extracellular matrix acts as crucial barrier, and communicates with the epidermis
to trigger protective responses following injury or infection. In C. elegans, we previously …

Transgenesis in model organisms is an essential tool for determining the function of protein-
coding genes and non-coding regulatory regions. In Caenorhabditis elegans, injected DNA …