Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

The limited per-pixel bandwidth of most microscopy methods requires compromises
between field of view, sampling density and imaging speed. This limitation constrains …

While calcium imaging has become a mainstay of modern neuroscience, the spectral
properties of current fluorescent calcium indicators limit deep-tissue imaging as well as …

Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary
objective to improve the resolution, field of view, and usability over previous variants. Owing …

The past decade has witnessed remarkable advances in the simultaneous measurement of
neuronal activity across many brain regions, enabling fundamentally new explorations of the …

Due to the size and opacity of vertebrate brains, it has until now been impossible to
simultaneously record neuronal activity at cellular resolution across the entire adult brain. As …

After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …