Background Tuberculosis (TB) incidence and mortality are declining worldwide; however,
poor detection of drug-resistant disease threatens to reverse current progress toward global …

Background The detection of mutations in the gyrA and gyrB genes in the Mycobacterium
tuberculosis genome that have been demonstrated to confer phenotypic resistance to …

The rise of antibiotic-resistant bacteria has led to an urgent need for rapid detection of drug
resistance in clinical samples, and improvements in global surveillance. Here we show how …

Rationale: The development of molecular diagnostics that detect both the presence of
Mycobacterium tuberculosis in clinical samples and drug resistance–conferring mutations …

The emergence of drug-resistant strains of Mycobacterium tuberculosis is a challenge to
global tuberculosis (TB) control. Although culture-based methods have been regarded as …

Background High-throughput DNA sequencing produces vast amounts of data, with millions
of short reads that usually have to be mapped to a reference genome or newly assembled …

We report the discovery and confirmation of 23 novel mutations with previously
undocumented role in isoniazid (INH) drug resistance, in catalase-peroxidase (katG) gene of …

Pyrazinamide (PZA) is an important first-line drug in the treatment of tuberculosis (TB) and of
significant interest to the HIV-infected community due to the prevalence of TB-HIV …

Background Accurate, comprehensive, and timely detection of drug-resistant tuberculosis
(TB) is essential to inform patient treatment and enable public health surveillance. This is …

Increasingly complex drug-resistant tuberculosis (DR-TB) is a major global health concern
and one of the primary reasons why TB is now the leading infectious cause of death …