Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive
approach for pathogen detection. The cost of high-throughput or next-generation …

The healthy lung was long thought of as sterile, but recent advances using molecular
sequencing approaches have detected bacteria at low levels. Healthy lung bacteria largely …

Nanopore sequencers can be used to selectively sequence certain DNA molecules in a pool
by reversing the voltage across individual nanopores to reject specific sequences, enabling …

The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …

Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …

Adaptive sampling is a method of software-controlled enrichment unique to nanopore
sequencing platforms. To test its potential for enrichment of rarer species within …

The application of sequencing technology is shifting from research to clinical laboratories
owing to rapid technological developments and substantially reduced costs. However …

Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …

Background Metagenomic next-generation sequencing (mNGS) has changed the diagnosis
landscape of lower respiratory tract infections (LRIs). With the development of newer …

Interactions between the developing microbiome and maturing immune system in early life
are critical for establishment of a homeostasis beneficial to both host and commensals. The …