Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Protein therapeutics have gained momentum in recent years and become a pillar in treating
many diseases and the only choice in several ailments. Protein therapeutics are highly …

Enzymatic peptide ligation holds great promise in the study of protein functions and
development of protein therapeutics. Owing to their high catalytic efficiency and a minimal …

Peptide asparaginyl ligases (PALs) are useful tools for precision modifications of proteins
and live‐cell surfaces by ligating peptides after Asn/Asp (Asx). They share high sequence …

Asparaginyl endopeptidases (AEPs) are cysteinyl enzymes naturally catalyzing the
hydrolysis and transpeptidation reactions at Asx–Xaa bonds. These reactions go by a …

Peptides and peptide aptamers have emerged as promising molecules for a wide range of
biomedical applications due to their unique properties and versatile functionalities. The …

Butelase-1 is an efficient ligase from Clitoria ternatea with wide applications in the food and
biopharmaceutical fields. This research aimed to achieve high-efficiency expression of …

Tumor necrosis factor-related apoptosis ligand (TRAIL) is a promising antitumor agent
candidate for its selective proapoptotic activity to various tumor cells. However, TRAIL …

Peptidyl asparaginyl ligases (PALs) are powerful tools for peptide macrocyclization. Herein,
we report that a derivative of Asn, namely Nγ‐hydroxyasparagine or Asn (OH), is an …

Asparaginyl endopeptidases (AEPs) or legumains are Asn/Asp (Asx)-specific proteases that
break peptide bonds, but also function as peptide asparaginyl ligases (PALs) that make …