Abstract Development of successful production strategies for recombinant proteins is a
challenging task due to problems associated with the scale‐up of bioprocesses. Recent …

Scorpion envenomation is a serious health problem in tropical and subtropical zones. The
access to scorpion antivenom is sometimes limited in availability and specificity. The …

Escherichia coli still serves as a beloved workhorse for the production of many
biopharmaceuticals as it fulfills essential criteria, such as having fast doubling times …

The present work elucidates the production of recombinant human asparaginase (rhASP)
under optimized fermentation and downstream processes in Escherichia coli. The maximum …

Multiple E. coli cultivations, producing recombinant proteins, lead to the formation of
inclusion bodies (IBs). IBs historically were considered as nondesired by-products, due to …

Background Recombinant protein production and purification from Escherichia coli is often
accompanied with expensive and complicated procedures, especially for therapeutic …

Aggregation of recombinant proteins into inclusion bodies (IBs) is the major drawback of
heterologous expression in Escherichia coli. Here, we evaluated the effects of a pH shift …

Abstract Background Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or
coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance …

Abstract Fibroblast growth factor-21 (FGF-21) is a potential cytokine for type II diabetes
mellitus. This study aimed to optimize recombinant human FGF-21 (rhFGF-21) production in …

Granulocyte colony-stimulating factor (G-CSF) is involved in generation of colonies
hematopoietic cells by stimulation of bone marrow cells, resulting in the survival …