Mass spectrometry‐based high‐throughput proteomics and its role in biomedical studies and systems biology
There are multiple reasons why the next generation of biological and medical studies
require increasing numbers of samples. Biological systems are dynamic, and the effect of a …
require increasing numbers of samples. Biological systems are dynamic, and the effect of a …
Phosphoproteomics in the age of rapid and deep proteome profiling
Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …
array of cellular processes. Because this modification serves as a rapid and reversible …
A compact quadrupole-orbitrap mass spectrometer with FAIMS interface improves proteome coverage in short LC gradients
DB Bekker-Jensen, A Martínez-Val… - Molecular & Cellular …, 2020 - ASBMB
State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and
their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per …
their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per …
Oncogenic KRAS regulates tumor cell signaling via stromal reciprocation
Oncogenic mutations regulate signaling within both tumor cells and adjacent stromal cells.
Here, we show that oncogenic KRAS (KRAS G12D) also regulates tumor cell signaling via …
Here, we show that oncogenic KRAS (KRAS G12D) also regulates tumor cell signaling via …
Heterocellular OSM-OSMR signalling reprograms fibroblasts to promote pancreatic cancer growth and metastasis
Pancreatic ductal adenocarcinoma (PDA) is a lethal malignancy with a complex
microenvironment. Dichotomous tumour-promoting and-restrictive roles have been ascribed …
microenvironment. Dichotomous tumour-promoting and-restrictive roles have been ascribed …
DYRK3 enables secretory trafficking by maintaining the liquid-like state of ER exit sites
The dual-specificity kinase DYRK3 controls the formation and dissolution of multiple
biomolecular condensates, regulating processes including stress recovery and mitotic …
biomolecular condensates, regulating processes including stress recovery and mitotic …
R2‐P2 rapid‐robotic phosphoproteomics enables multidimensional cell signaling studies
M Leutert, RA Rodríguez‐Mias, NK Fukuda… - Molecular systems …, 2019 - embopress.org
Recent developments in proteomics have enabled signaling studies where> 10,000
phosphosites can be routinely identified and quantified. Yet, current analyses are limited in …
phosphosites can be routinely identified and quantified. Yet, current analyses are limited in …
Chemokine CXCL4 interactions with extracellular matrix proteoglycans mediate widespread immune cell recruitment independent of chemokine receptors
Leukocyte recruitment from the vasculature into tissues is a crucial component of the
immune system but is also key to inflammatory disease. Chemokines are central to this …
immune system but is also key to inflammatory disease. Chemokines are central to this …
Wip1 inhibits neutrophil extracellular traps to promote abscess formation in mice by directly dephosphorylating Coronin-1a
Y Chen, C Zhao, H Guo, W Zou, Z Zhang… - Cellular & Molecular …, 2023 - nature.com
Neutrophil extracellular traps (NETs) participate in the rapid inhibition and clearance of
pathogens during infection; however, the molecular regulation of NET formation remains …
pathogens during infection; however, the molecular regulation of NET formation remains …
Human RIF 1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation
The human RIF 1 protein controls DNA replication, but the molecular mechanism is largely
unknown. Here, we demonstrate that human RIF 1 negatively regulates DNA replication by …
unknown. Here, we demonstrate that human RIF 1 negatively regulates DNA replication by …