DNA topoisomerases are a diverse set of essential enzymes responsible for maintaining
chromosomes in an appropriate topological state. Although they vary considerably in …

Topoisomerase II is a ubiquitous enzyme that is essential for the survival of all eukaryotic
organisms and plays critical roles in virtually every aspect of DNA metabolism. The enzyme …

Adriamycin (doxorubicin), a potent antitumor drug in clinical use, interacts with nucleic acids
and cell membranes, but the molecular basis for its antitumor activity is unknown. Similar to …

We have constructed five new temperature-sensitive DNA topoisomerase II mutations and
have analyzed their physiological consequences in yeast. Several lines of evidence suggest …

We have obtained a polyclonal antibody that recognizes a major polypeptide component of
chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr …

Topoisomerase 11 is an essential enzyme that plays a role in virtually every cellular DNA
process. This enzyme interconverts different topological forms of DNA by passing one …

Topotecan-or mitoxantrone-selected cell lines (T8 and MX3, respectively), derived from the
human IGROV1 ovarian cancer cell line, were resistant to the topoisomerase I inhibitors …

Abstract Treatment of human HL-60 or KG1A leukemia cells with the topoisomerase II
inhibitor etoposide resulted in extensive DNA degradation. When DNA integrity was …

Many intercalative antitumor drugs have been shown to cleave DNA indirectly through their
specific effect on the stabilization of a cleavable complex formed between mammalian DNA …

Using the P4 unknotting assay, DNA topoisomerase II has been purified from several
mammalian cells. Similar to prokaryotic DNA gyrase, mammalian DNA topoisomerase II can …