Nearly 30% of currently approved recombinant therapeutic proteins are produced in
Escherichia coli. Due to its well-characterized genetics, rapid growth and high-yield …

Human interferon gamma is a cytokine belonging to a diverse group of interferons which
have a crucial immunological function against mycobacteria and a wide variety of viral …

This paper reviews recent strategies used for increasing specific yield and productivity in
high cell density cultures. High cell density cultures offer an efficient means for the …

Understanding the growth characteristics of microorganisms is an essential step in
bioprocessing, not only because product formation may be growth-associated but also …

Removal of endotoxins from recombinant proteins is a critical and challenging step in the
preparation of injectable therapeutics, as endotoxin is a natural component of the bacterial …

This work proposes a hybrid observer based on an asymptotic observer combined with an
extended Kalman filter applied to high-density cultures of S. cerivisiae. The observer …

Escherichia coli is commonly used industrially to manufacture recombinant proteins for
biopharmaceutical applications, as well as in academic and industrial settings for R&D …

A common method to minimize overflow metabolism and to enable high cell-density is to
operate microbial processes in fed-batch mode under carbon-limiting conditions. This …

Abstract “Giving the cells exactly what they need, when they need it” is the core idea behind
the proposed bioprocess control strategy: operating bioprocess based on the physiological …

Sažetak Microbial fermentation processes are of great importance for the production of many
bioproducts. Even for established processes, improvements in product yield, productivity …