CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Synthetic lethality (SL) provides a conceptual framework for tackling targets that are not
classically “druggable,” including loss-of-function mutations in tumor suppressor genes …

The ability to control gene expression and generate quantitative phenotypic changes is
essential for breeding new and desired traits into crops. Here we report an efficient, facile …

Filamentous fungi are critical in the transition to a more sustainable food system. While
genetic modification of these organisms has promise for enhancing the nutritional value …

Sorting large libraries of cells for improved small molecule secretion is throughput limited.
Here, we combine producer/secretor cell libraries with whole-cell biosensors using a …

CRISPR-Cas9 functional genomic screens uncover gene targets linked to various
phenotypes for metabolic engineering with remarkable efficiency. However, these genome …

The well-designed microbial cell factory finds wide applications in chemical, pharmaceutical,
and food industries due to its sustainable and environmentally friendly features. Recently …

Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables
programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene …

A lack of high-throughput techniques for making titrated, gene-specific changes in
expression limits our understanding of the relationship between gene expression and cell …

Numerous applications of noncanonical amino acids (ncAAs) in basic biology and
therapeutic development require efficient protein biosynthesis using an expanded genetic …