▪ Abstract A decade after the discovery of electrospray and matrix-assisted laser desorption
ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules …

Phosphorylation of light-harvesting chlorophyll a/b proteins (LHC II) and PSII core proteins,
and its regulation by thylakoid protein kinases and protein phosphatase is reviewed. The …

Mammalian cells have about 30,000 times as many protein molecules as mRNA molecules,
which has major implications in the development of proteomics technologies. We discuss …

A method to correlate the uninterpreted tandem mass spectra of peptides produced under
low energy (10–50 eV) collision conditions with amino acid sequences in the Genpept …

Abstract Interleukin-1β (IL-1β)-converting enzyme cleaves the IL-1β precursor to mature IL-
1β, an important mediator of inflammation. The identification of the enzyme as a unique …

A method to correlate uninterpreted tandem mass spectra of modified peptides, produced
under low-energy (10-50 eV) collision conditions, with amino acid sequences in a protein …

Mitogen‐activated protein kinase (MAP kinase) is a 42 kd serine/threonine protein kinase
whose enzymatic activity requires phosphorylation of both tyrosyl and threonyl residues. As …

Aspects of the development of mass spectrometry over the past three decades are briefly
reviewed and growth points in the subject are identified. Molecular imaging by mass …

Class III beta-tubulin, isolated from adult bovine brain, is resolved into at least seven charge
variants on isoelectric focusing gels. To identify the posttranslational modifications …

Subfemtomole peptide sequence analysis has been achieved using microcapillary HPLC
columns, with integrated nanoelectrospray emitters, coupled directly to a Fourier transform …