Acute stress puts cells at risk, and rapid adaptation is crucial for maximizing cell survival.
Cellular adaptation mechanisms include modification of certain aspects of cell physiology …

Mathematical modeling of biological processes provides deep insights into complex cellular
systems. While quantitative and continuous models such as differential equations have been …

Transcription factors (TFs) regulate gene programs, thereby controlling diverse cellular
processes and cell states. To comprehensively understand TFs and the programs they …

Genetic screens help infer gene function in mammalian cells, but it has remained difficult to
assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop …

The expression of co-inhibitory receptors, such as CTLA-4 and PD-1, on effector T cells is a
key mechanism for ensuring immune homeostasis. Dysregulated expression of co-inhibitory …

An appropriate response and adaptation to hyperosmolarity, ie., an external osmolarity that
is higher than the physiological range, can be a matter of life or death for all cells. It is …

To obtain rates of mRNA synthesis and decay in yeast, we established dynamic
transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with …

Understanding the principles governing mammalian gene regulation has been hampered by
the difficulty in measuring in vivo binding dynamics of large numbers of transcription factors …

Dynamic protein binding to DNA elements regulates genome function and cell fate. Although
methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect …

The earliest stages of development in most metazoans are driven by maternally deposited
proteins and mRNAs, with widespread transcriptional activation of the zygotic genome …