Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing

AM Azevedo, PAJ Rosa, IF Ferreira… - Trends in …, 2009 - cell.com
The therapeutic use of proteins has created an increasing demand for feasible and
economical methods for both up-and downstream processes. However, whereas upstream …

Continuous aqueous two-phase systems devices for the recovery of biological products

E Espitia-Saloma, P Vázquez-Villegas, O Aguilar… - Food and Bioproducts …, 2014 - Elsevier
Aqueous two-phase systems (ATPS) have proved to be a suitable technique for the recovery
of biological products. Although ATPS have been in the field of primary recovery and …

Optimal Design of Aqueous Two-Phase Systems for Biomolecule Partitioning

Y Chen, X Liang, GM Kontogeorgis - Industrial & Engineering …, 2023 - ACS Publications
Aqueous two-phase systems (ATPS) have exhibited superior performance in many
biotechnological applications. To promote the implementation of these powerful platforms by …

Liquid–liquid extraction of metal ions without use of organic solvent

GD Rodrigues, MCH da Silva, LHM da Silva… - Separation and …, 2008 - Elsevier
The extraction behavior of the ions Ni (II), Fe (III), Cd (II), Co (II), Zn (II) and Cu (II) was
studied as a function of the amount of extractant added, in aqueous two-phase systems …

[HTML][HTML] Artificial neural network modeling on the polymer-electrolyte aqueous two-phase systems involving biomolecules

Y Chen, X Liang, GM Kontogeorgis - Separation and Purification …, 2023 - Elsevier
Polymer-electrolyte aqueous two-phase systems (ATPS) have demonstrated their superior
performance in the separation and purification of high-value biomolecules. However, these …

Copper recovery from ore by liquid–liquid extraction using aqueous two-phase system

LR de Lemos, IJB Santos, GD Rodrigues… - Journal of hazardous …, 2012 - Elsevier
We investigated the extraction behavior of Cu (II) in the aqueous two-phase system (ATPS)
formed by (L35+ MgSO4+ H2O) or (L35+ (NH4) 2SO4+ H2O) in the presence of the …

Aqueous two-phase systems: an efficient, environmentally safe and economically viable method for purification of natural dye carmine

AB Mageste, LR de Lemos, GMD Ferreira… - … of Chromatography A, 2009 - Elsevier
Partition of the natural dye carmine has been studied in aqueous two-phase systems
prepared by mixing aqueous solutions of polymer or copolymer with aqueous salt solutions …

Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aqueous two-phase systems (PEG/sulfate)

TP Nascimento, AE Sales, CS Porto… - … of Chromatography B, 2016 - Elsevier
A fibrinolytic protease from M. subtilissimus UCP 1262 was recovered and partially purified
by polyethylene glycol (PEG)/sodium sulfate aqueous two-phase systems (ATPS). The …

Study of curcumin partitioning in polymer-salt aqueous two phase systems

HS Shiran, M Baghbanbashi, FG Ahsaie… - Journal of Molecular …, 2020 - Elsevier
Curcumin is a polyphenolic compound derived from Curcuma longa, which has a wide
range of pharmacological applications and it constitutes 2–5% of turmeric. An efficient …

[HTML][HTML] Using three-phase partitioning for the purification and recovery of antibodies from biological media

EV Capela, I Magnis, AFCS Rufino… - Separation and …, 2023 - Elsevier
Antibodies, in particular immunoglobulin G (IgG), are one of the biopharmaceutical industry
highflyers, with relevance for the treatment of several diseases. However, the recovery of …