The production of proteins in sufficient amounts is key for their study or use as biotherapeutic
agents. Escherichia coli is the host of choice for recombinant protein production given its fast …

All cellular processes are the results of synchronized actions of several intracellular
biochemical pathways. Recent emphasis is to visualize such pathways using appropriate …

Differential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical
technique that has seen many applications over the years, ranging from protein folding state …

Elastin-like polypeptides (ELPs) are stimulus-responsive biopolymers derived from human
elastin. Their unique properties—including lower critical solution temperature phase …

Graphene has demonstrated great potential in next-generation electronics due to its unique
two-dimensional structure and properties including a zero-gap band structure, high electron …

The ease of genetic manipulation, low cost, rapid growth and number of previous studies
have made Escherichia coli one of the most widely used microorganism species for …

In the two decades since the introduction of the “click chemistry” concept, the toolbox of “click
reactions” has continually expanded, enabling chemists, materials scientists, and biologists …

Pichia pastoris, a methylotrophic yeast, is an established system for the production of
heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise …

Western blotting is a technique that has been in practice for more than three decades that
began as a means of detecting a protein target in a complex sample. Although there have …

Recent years have seen intensive progress in measuring protein translation. However, the
contributions of coding sequences to the efficiency of the process remain unclear. Here, we …