The gene of transcriptional repressor GATA binding 1 (TRPS1), as an atypical GATA
transcription factor, has received considerable attention in a plethora of physiological and …

Simple Summary For many decades, the cellular and molecular mechanisms that
orchestrate the differentiation of epithelial subtypes in the mammary gland have been a …

Metastatic breast-cancer is a major cause of death in women worldwide, yet the relationship
between oncogenic drivers that promote metastatic versus primary cancer is still …

Detection of single-based mutation (SbM), which is of ultra-low abundance against wild-type
alleles, are typically constrained by the level of multiplexing, sensitivity for single-base …

Functionally characterizing the genetic alterations that drive pancreatic cancer is a
prerequisite for precision medicine. Here, we perform somatic CRISPR/Cas9 mutagenesis …

Mutations in the gene ankyrin repeat domain containing 11 (ANKRD11/ANCO1) play a role
in neurodegenerative disorders, and its loss of heterozygosity and low expression are seen …

An important goal in cancer research is to identify driver genes and mutations. Reasoning
that such mutations often alter enzymatic functions, we investigated the cancer driver role of …

The identification of cancer maintenance genes—driver genes essential to tumor survival—
is fundamental for developing effective cancer therapy. Transposon-based insertional …

Chromosome instability is highly prevalent in cancer and drives large scale chromosomal
imbalances, known as aneuploidies. However, how aneuploidy contributes to tumorigenesis …

In this thesis, I describe my efforts to utilize high-throughput (HTP) functional genomics
approaches to better characterize how genotypes can influence phenotypes in diploid …