Historically, the discovery of the helical structure of DNA by Watson and Crick1 provided the
impetus to attempt the challenging synthesis of oligonucleotides of defined sequences …

Improvements in the synthesis, deprotection and purification of oligoribonucleotides are
described. These advances allow for reduced synthesis and deprotection times, while …

This volume is a timely and comprehensive description of the many facets of DNA and RNA
modification-editing processes and to some extent repair mechanisms. Each chapter offers …

The biological potential of RNA along with the variety of possible applications has led to an
increased demand for large-scale production of RNA molecules. Recent developments in …

A series of nucleobase-modified siRNA duplexes containing “rare” nucleosides, 2-
thiouridine (s2U), pseudouridine (Ψ), and dihydrouridine (D), were evaluated for their …

Expansion of the genetic alphabet by an unnatural base pair system provides a powerful
tool for modern biotechnology. As an alternative to previous unnatural base pairs, we have …

The complete chemical synthesis of an E. coli tRNA Ala with its specific minor nucleosides,
dlhydrouridine, ribothymidine and pseudouridine, is reported. The method makes use of …

Modified nucleotides can be incorporated site specifically into RNA by the use of total
chemical synthesis as well as by use of a variety of recombinant RNA techniques. The range …

Disclosed herein are protecting groups for exocyclic amino groups of the bases adenine,
guanine and cytosine for use in the synthesis of oligonucleotides, the protect ing groups …

Synthetic oligoribonucleotides have been used to probe the interaction of MS2 coat protein
with the translational operator of the MS2 replicase gene. We have investigated the possible …