Cell cultures are important material of study for the variety of advantages that they offer. Both
established continuous cell lines and primary cell cultures continue to be invaluable for …

The use of animal models in drug discovery studies presents issues with feasibility and
ethical concerns. To address these limitations, in vitro tissue models have been developed …

Purpose. The purpose of this review is to provide an overview of the principles of and
barriers to drug transport and delivery to solid tumors. Methods. This review consists of four …

Purpose: To give a state-of-the-art overview on the promise of three-dimensional (3-D)
culture systems for anticancer drug development, with particular emphasis on multicellular …

Research on resistance to cancer treatment was mainly focused for 20 years on multidrug
resistance (MDR). No useful method of reversing MDR, suitable for clinical use, has yet …

Cancer treatment regimens often include multiple anticancer agents targeting different
cellular mechanisms in delicate balance with associated toxicity. Drug delivery systems offer …

Recent studies on paclitaxel (Taxol), a microtubule‐stabilizing agent and effective anti‐
cancer drug, have identified numerous cellular and molecular effects, such as induction of …

Creating multicellular tumor spheroids is critical for characterizing anticancer treatments
since it may provide a better model than monolayer culture of in vivo tumors. Moreover …

Cell-based assays are more complex than cell-free test systems but still reflect a highly
artificial cellular environment. Incorporation of organotypic 3-dimensional (3-D) culture …

Despite their simplicity, monolayer cell cultures are not able to accurately predict drug
behavior in vivo due to their inability to accurately mimic cell-cell and cell-matrix interactions …