Molecular cloning is a cornerstone of biomedical, biotechnological, and synthetic biology
research. As such, improved cloning methodologies can significantly advance the speed …

The bacterial pathogen Pseudomonas aeruginosa activates expression of many virulence
genes in a cell density-dependent manner by using an intricate quorum-sensing (QS) …

The assembly of DNA fragments with homologous arms is becoming popular in routine
cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for …

Genomically minimal cells, such as JCVI-syn3. 0, offer a platform to clarify genes underlying
core physiological processes. Although this minimal cell includes genes essential for …

In vivo homologous recombination holds the potential for optimal molecular cloning,
however, current strategies require specialised bacterial strains or laborious protocols. Here …

Background The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9)
has been considerably decreased by rationally engineered variants with increased fidelity …

Many species of proteobacteria communicate with kin and coordinate group behaviors
through a form of cell-cell signaling called acyl-homoserine lactone (AHL) quorum sensing …

The opportunistic pathogen Pseudomonas aeruginosa is a leading cause of airway infection
in cystic fibrosis (CF) patients. P. aeruginosa employs several hierarchically arranged and …

The chloroplast is an essential organelle in microalgae for conducting photosynthesis, thus
enabling the photoautotrophic growth of microalgae. In addition to photosynthesis, the …

Crh proteins catalyze crosslinking of chitin and glucan polymers in fungal cell walls. Here,
we show that the BcCrh1 protein from the phytopathogenic fungus Botrytis cinerea acts as a …