Recent developments in capillary isoelectric focusing

LHH Silvertand, JS Torano, WP Van Bennekom… - … of Chromatography A, 2008 - Elsevier
The developments in capillary isoelectric focusing (cIEF) over the period 2003–2007 are
reviewed. With the focus on technological aspects, cIEF papers published in the fields of …

Ultramicro enzyme assays in a capillary electrophoretic system

J Bao, FE Regnier - Journal of Chromatography A, 1992 - Elsevier
This paper describes an ultramicro method for achieving enzyme assays. Enzyme saturating
concentrations of substrate, coenzyme when appropriate, and running buffer were mixed …

Capillary isoelectric focusing as a tool in the examination of antibodies, peptides and proteins of pharmaceutical interest

X Liu, Z Sosic, IS Krull - Journal of Chromatography A, 1996 - Elsevier
This paper describes the recent history and development of capillary isoelectric focusing
(cIEF), as it has evolved over the past 10 years forming a distinct mode of high-performance …

Capillary isoelectric focusing with a universal concentration gradient imaging system using a charge-coupled photodiode array

J Wu, J Pawliszyn - Analytical Chemistry, 1992 - ACS Publications
The focused patterns of protein samples by capillary Isoelectric focusing (CIEF) were
monitored without mobilization by a concentration gradient Imaging detection system based …

[图书][B] Capillary electrophoresis of proteins

T Wehr, R Rodriguez-Diaz, M Zhu - 1998 - taylorfrancis.com
" Provides practical information on the application of capillary electrophoresis (CE) to protein
analysis, with an emphasis on developing and optimizing CE techniques in the laboratory …

Dual detection for capillary isoelectric focusing with refractive index gradient and absorption imaging detectors

J Wu, J Pawliszyn - Analytical Chemistry, 1994 - ACS Publications
A unique imaging detector was constructed for capillary isoelectric focusing (CIEF) by
combining a selective optical absorption imaging detector with a universal refractive index …

Current trends in capillary isoelectric focusing of proteins

PG Righetti, C Gelfi, M Conti - Journal of Chromatography B: Biomedical …, 1997 - Elsevier
Isoelectric focusing (IEF) in thin capillaries is reviewed here. After an introduction on the
genesis and chemistry of the carrier ampholyte buffers, different approaches to IEF are …

Hologram-based refractive index detector for capillary electrophoresis: separation of metal ions

B Krattiger, GJM Bruin, AE Bruno - Analytical Chemistry, 1994 - ACS Publications
A novel refractive index detector suitable for capillary sepa-rations is presented and
demonstrated in the capillary elec-trophoretic separation of metal cations in 10-itm-id tubes …

Apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems

JB Pawliszyn - US Patent 5,395,502, 1995 - Google Patents
2. State of the Art It has been known for some time that a refractive index gradient such as
produced by a concentration gradient in a fluid such as a gas, liquid, or supercritical fluid …

Laser-based non-fluorescence detection techniques for liquid separation systems

T De Beer, NH Velthorst, UAT Brinkman… - … of Chromatography A, 2002 - Elsevier
Over the last two decades, the possibility to use lasers for detection purposes in column
liquid chromatography (LC) and capillary electrophoresis (CE) received much attention in …