The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc
building blocks are available at low cost because of the economies of scale arising from …

Selective modification of native proteins in live cells is one of the central challenges in recent
chemical biology. As a unique bioorthogonal approach, ligand-directed chemistry recently …

It is shown for the first time that N-sulfonyl amides can be efficiently prepared by an
unconventional approach of the hydrative reaction between terminal alkynes, sulfonyl …

The covalent inhibition of a target protein has gained widespread attention in the field of
drug discovery. Most of the current covalent drugs utilize the high reactivity of cysteines …

Peptide thioesters play a key role in convergent protein synthesis strategies such as native
chemical ligation, traceless Staudinger ligation, and Ag+‐mediated thioester ligation. The …

The naturally-occurring cyclic cystine-knot microprotein trypsin inhibitors MCoTI-I and MCoTI-
II have been synthesised using both thia-zip native chemical ligation and a biomimetic …

Catalyst-mediated protein modification is a powerful approach for the imaging and
engineering of natural proteins. We have previously developed affinity-guided 4 …

The chemical synthesis of peptides or small proteins is often an important step in many
research projects and has stimulated the development of numerous chemical …

Conspectus Selective chemical modification of endogenous proteins in living systems with
synthetic small molecular probes is a central challenge in chemical biology. Such …