Comparison of iSeq and MiSeq as the two platforms for 16S rRNA sequencing in the study of the gut of rat microbiome

D Salamon, B Zapała, A Krawczyk… - Applied Microbiology …, 2022 - Springer
Amplicon-based next-generation sequencing (NGS) of the 16S ribosomal RNA (16S)
regions is a culture-free method used to identify and analyze Procaryota occurring within a …

Reference-free plant disease detection using machine learning and long-read metagenomic sequencing

MA Johnson, BA Vinatzer, S Li - Applied and Environmental …, 2023 - Am Soc Microbiol
Surveillance for early disease detection is crucial to reduce the threat of plant diseases to
food security. Metagenomic sequencing and taxonomic classification have recently been …

Recent research accomplishments on early detection of Xylella fastidiosa outbreaks in the Mediterranean Basin

MP Velasco-Amo, A Vicent, PJ Zarco-Tejada… - Phytopathologia …, 2022 - torrossa.com
Xylella fastidiosa is a major transboundary plant pest, causing severe socioeconomic
impacts. Development of preventive strategies and methods for surveillance, early detection …

Century-old herbarium specimen provides insights into Pierce's disease of grapevines emergence in the Americas

MA Donegan, AK Kahn, N Becker, AC Siri, PE Campos… - Current Biology, 2025 - cell.com
Fossils and other preserved specimens are integral for informing timing and evolutionary
history in every biological system. By isolating a plant pathogen genome from herbarium …

Concept note: Toward metagenomic sequencing for rapid, sensitive, and accurate detection of bacterial pathogens in potato seed production

V Roman-Reyna, R Rioux, B Babler, T Klass… - PhytoFrontiers …, 2023 - Am Phytopath Society
Disease-free planting stocks and formal certification systems are critical for the profitable
production of vegetatively propagated crops. Propagation through seed tubers is the …

Live tracking of a plant pathogen outbreak reveals rapid and successive, multidecade plasmid reduction

V Roman-Reyna, A Sharma, H Toth, Z Konkel… - Msystems, 2024 - Am Soc Microbiol
Quickly understanding the genomic changes that lead to pathogen emergence is necessary
to launch mitigation efforts and reduce harm. In this study, we tracked in real time a 2022 …

A Survey of Xylella fastidiosa in the US State of Virginia Reveals Wide Distribution of Both Subspecies fastidiosa and multiplex in Grapevine

S Abdelrazek, E Bush, C Oliver, H Liu… - …, 2024 - Am Phytopath Society
Global travel and trade in combination with climate change are expanding the geographic
distribution of plant pathogens. The bacterium Xylella fastidiosa is a prime example. Native …

Draft Genome Sequence Resource of Xylella fastidiosa Strain Alm_Lz_1 Associated with a New Outbreak in Lazio, Italy

A Giampetruzzi, G Loconsole, S Zicca… - …, 2023 - Am Phytopath Society
In November 2021, in the framework of the annual surveillance program implemented in all
European Union countries, an outbreak of Xylella fastidiosa was intercepted in the Italian …

Evaluation of Metabarcoding Methods for Plant Disease Surveillance

EA Newberry, S Srivastava, SO Nunziata… - …, 2023 - Am Phytopath Society
Metabarcoding holds great potential for general plant pathogen surveillance by providing an
untargeted profile of the host microbiome. However, the standard marker utilized for …