The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …

Biocatalysis has become an important aspect of modern organic synthesis, both in
academia and across the chemical and pharmaceutical industries. Its success has been …

Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic
amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow …

Thermostability is considered to be an important parameter to measure the feasibility of
enzymes for industrial applications. Generally, higher thermostability makes an enzyme …

The emergence of robust methods to expand the genetic code allows incorporation of non-
canonical amino acids into the polypeptide chain of proteins, thus making it possible to …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Interactions among biomacromolecules, predominantly noncovalent, underpin biological
processes. However, recent advancements in biospecific chemistry have enabled the …

A deaminative reductive coupling of amino acid pyridinium salts with aryl bromides has
been developed to enable efficient synthesis of noncanonical amino acids and …

In recent years, powerful genetic code reprogramming methods have emerged that allow
new functional components to be embedded into proteins as noncanonical amino acid …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …