Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …

Decades of research have resulted in a remarkably detailed understanding of the molecular
mechanisms of bacterial DNA replication, transcription and translation. Our understanding of …

It has been assumed that DNA synthesis by the leading-and lagging-strand polymerases in
the replisome must be coordinated to avoid the formation of significant gaps in the nascent …

The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli,
a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular …

The Escherichia coli DNA replication machinery has been used as a road map to uncover
design rules that enable DNA duplication with high efficiency and fidelity. Although the …

Abstract The bacterial RecF, RecO, and RecR proteins are an epistasis group involved in
loading RecA protein into post-replication gaps. However, the targeting mechanism that …

Limited experimental tools are available to study the consequences of collisions between
DNA-bound molecular machines. Here, we repurpose a catalytically inactivated Cas9 …

Genome duplication occurs while the template DNA is bound by numerous DNA-binding
proteins. Each of these proteins act as potential roadblocks to the replication fork and can …

DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …

The molecular machinery responsible for DNA replication, the replisome, must efficiently
coordinate DNA unwinding with priming and synthesis to complete duplication of both …