Current challenges and best-practice protocols for microbiome analysis

R Bharti, DG Grimm - Briefings in bioinformatics, 2021 - academic.oup.com
Analyzing the microbiome of diverse species and environments using next-generation
sequencing techniques has significantly enhanced our understanding on metabolic …

Benchmarking metagenomics tools for taxonomic classification

HY Simon, KJ Siddle, DJ Park, PC Sabeti - Cell, 2019 - cell.com
Metagenomic sequencing is revolutionizing the detection and characterization of microbial
species, and a wide variety of software tools are available to perform taxonomic …

Inference and reconstruction of the heimdallarchaeial ancestry of eukaryotes

L Eme, D Tamarit, EF Caceres, CW Stairs, V De Anda… - Nature, 2023 - nature.com
In the ongoing debates about eukaryogenesis—the series of evolutionary events leading to
the emergence of the eukaryotic cell from prokaryotic ancestors—members of the Asgard …

Improved metagenomic analysis with Kraken 2

DE Wood, J Lu, B Langmead - Genome biology, 2019 - Springer
Although Kraken'sk-mer-based approach provides a fast taxonomic classification of
metagenomic sequence data, its large memory requirements can be limiting for some …

A chromosome conformation capture ordered sequence of the barley genome

M Mascher, H Gundlach, A Himmelbach, S Beier… - Nature, 2017 - nature.com
Cereal grasses of the Triticeae tribe have been the major food source in temperate regions
since the dawn of agriculture. Their large genomes are characterized by a high content of …

Ranking the biases: The choice of OTUs vs. ASVs in 16S rRNA amplicon data analysis has stronger effects on diversity measures than rarefaction and OTU identity …

M Chiarello, M McCauley, S Villéger, CR Jackson - PloS one, 2022 - journals.plos.org
Advances in the analysis of amplicon sequence datasets have introduced a methodological
shift in how research teams investigate microbial biodiversity, away from sequence identity …

A review of methods and databases for metagenomic classification and assembly

FP Breitwieser, J Lu, SL Salzberg - Briefings in bioinformatics, 2019 - academic.oup.com
Microbiome research has grown rapidly over the past decade, with a proliferation of new
methods that seek to make sense of large, complex data sets. Here, we survey two of the …

Robust taxonomic classification of uncharted microbial sequences and bins with CAT and BAT

FAB Von Meijenfeldt, K Arkhipova, DD Cambuy… - Genome biology, 2019 - Springer
Current-day metagenomics analyses increasingly involve de novo taxonomic classification
of long DNA sequences and metagenome-assembled genomes. Here, we show that the …

Critical assessment of metagenome interpretation—a benchmark of metagenomics software

A Sczyrba, P Hofmann, P Belmann, D Koslicki… - Nature …, 2017 - nature.com
Methods for assembly, taxonomic profiling and binning are key to interpreting metagenome
data, but a lack of consensus about benchmarking complicates performance assessment …

Fast and sensitive taxonomic classification for metagenomics with Kaiju

P Menzel, KL Ng, A Krogh - Nature communications, 2016 - nature.com
Metagenomics emerged as an important field of research not only in microbial ecology but
also for human health and disease, and metagenomic studies are performed on increasingly …