ORBIT for E. coli: kilobase-scale oligonucleotide recombineering at high throughput and high efficiency
SH Saunders, AM Ahmed - Nucleic Acids Research, 2024 - academic.oup.com
Microbiology and synthetic biology depend on reverse genetic approaches to manipulate
bacterial genomes; however, existing methods require molecular biology to generate …
bacterial genomes; however, existing methods require molecular biology to generate …
Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients
In 2019, the novel SARS-CoV-2 coronavirus emerged in China, causing the pneumonia
named COVID-19. At the beginning, all research efforts were focused on the spike (S) …
named COVID-19. At the beginning, all research efforts were focused on the spike (S) …
No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum
AM Diaz-Garza, N Merindol, KCG Dos Santos… - Microbial Cell …, 2024 - Springer
Background Conjugation-based episome delivery is a highly efficient method used to
transfer DNA into the diatom Phaeodactylum tricornutum, facilitating the production of …
transfer DNA into the diatom Phaeodactylum tricornutum, facilitating the production of …
Resistance mechanism of Escherichia coli strains with different ampicillin resistance levels
O Türkyılmaz, C Darcan - Applied Microbiology and Biotechnology, 2024 - Springer
Antibiotic resistance is an important problem that threatens medical treatment. Differences in
the resistance levels of microorganisms cause great difficulties in understanding the …
the resistance levels of microorganisms cause great difficulties in understanding the …
Efficient modification and preparation of circular DNA for expression in cell culture
RT Oliynyk, GM Church - Communications Biology, 2022 - nature.com
DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell
culture experiments. The preparation of plasmids typically involves a laborious process of …
culture experiments. The preparation of plasmids typically involves a laborious process of …
Directed Evolution of Silicatein Reveals Biomineralization Synergism between Protein Sequences
TN Vigil, MJC Rowson, AJ Frost, AR Janiga… - ACS …, 2025 - ACS Publications
Biomineralization is a green synthesis route for a variety of metal nanoparticles. Silicatein is
a biomineralization protein originally found in marine sponge Tethya aurantia that converts …
a biomineralization protein originally found in marine sponge Tethya aurantia that converts …
Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL)
M Wäneskog, EE Hoch-Schneider, S Garg… - mBio, 2024 - journals.asm.org
High-throughput DNA transformation techniques are invaluable when generating high-
diversity mutant libraries, a cornerstone of successful protein engineering. However …
diversity mutant libraries, a cornerstone of successful protein engineering. However …
[HTML][HTML] CRISPR/Cas-Mediated Knockdown of PD-L1 and KRAS in Lung Cancer Cells
SA Abounar, NA El-Nikhely, K Turkowski… - International Journal of …, 2024 - mdpi.com
Cancer cells can escape death and surveillance by the host immune system in various
ways. Programmed cell death ligand 1 (PD-L1) is a transmembrane protein that is …
ways. Programmed cell death ligand 1 (PD-L1) is a transmembrane protein that is …
Quantifying the fractal complexity of nutrient transport channels in Escherichia coli biofilms under varying cell shape and growth environment
B Bottura, L Rooney, M Feeney… - …, 2024 - microbiologyresearch.org
Recent mesoscopic characterization of nutrient-transporting channels in Escherichia coli
has allowed the identification and measurement of individual channels in whole mature …
has allowed the identification and measurement of individual channels in whole mature …
Heat pre-treatment reduces multiplicity of plasmid transformations in yeast during electroporation, without diminishing the transformation efficiency
M Wäneskog, EE Hoch-Schneider, S Garg… - bioRxiv, 2024 - biorxiv.org
High-throughput DNA transformation techniques are invaluable when creating high-diversity
mutant libraries, and the success rate of any protein engineering endeavors is directly …
mutant libraries, and the success rate of any protein engineering endeavors is directly …